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Lane 2 (both membranes): DPP9 antibody - Catalytic domain (ab42080) at 1/2000 dilution
Membrane 1 : Purified protein from cow testes with No Blocking Peptide
Membrane 2 : Purified protein from cow testes with with DPP9 peptide (Catalytic domain) (ab44599) at 1/1000 dilution
Lysates/proteins at 0.5 µg per lane.
Secondary
An HRP-conjugatee Goat polyclonal at 1/5000 dilution
Lane 1 (both membranes) Molecular weight marker
Blocking Step: 5% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Yannick Waumans
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