Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
DPP9 peptide (Catalytic domain)
See all DPP9 products (4) ...
Protein description
Synthetic peptide DPP9. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab42080 - DPP9 antibody - Catalytic domain.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
-
Western blot - DPP9 peptide (Catalytic domain) (ab44599)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (Dilution optimised using Chromogenic detection.)
ELISA: Use at an assay dependent dilution.
Application notes
This peptide can be used with studies using ab42080.
Protein info
Function
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.
Tissue specificity
Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain.
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily.
Cellular localization
Cytoplasm > cytosol.
Target information above from: UniProt accessionQ86TI2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
DPP9 peptide (Catalytic domain) images:
Western blot - DPP9 peptide (Catalytic domain) (ab44599)
Lane 2 (both membranes): DPP9 antibody - Catalytic domain (ab42080) at 1/2000 dilution
Membrane 1 : Purified protein from cow testes with No Blocking Peptide
Membrane 2 : Purified protein from cow testes with with DPP9 peptide (Catalytic domain) (ab44599) at 1/1000 dilution
Lysates/proteins at 0.5 µg per lane.
Secondary
An HRP-conjugatee Goat polyclonal at 1/5000 dilution
Lane 1 (both membranes) Molecular weight marker
Blocking Step: 5% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Yannick Waumans
References for DPP9 peptide (Catalytic domain) (ab44599)
ab44599 has not yet been referenced specifically in any publications.
Publishing research using ab44599? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"