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Overview

  • Product nameDR4 Human ELISA KitSee all DR4 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 475pg/ml 13 2.76%
    B 8 54pg/ml 4 7.57%
    Inter-assay
    Sample n Mean SD CV%
    A 12 498pg/ml 40 8.11%
    B 12 53pg/ml 7 13.87%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93.4 250pg/ml - 1000pg/ml

  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human DR4 ELISA is to be used for the in-vitro quantitative determination of TNF-related apoptosis-inducing-ligand Receptor 1 (DR4) in human serum, buffered solutions, cell culture medium or cell lysate. The assay will recognize both natural and recombinant human DR4. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The DR4 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations and unknowns are pipetted into these wells. During the first incubation, the DR4 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for DR4 is incubated. Then the enzyme ( streptavidin-horse radish peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of DR4 present in the samples.

     

    Recognizes Human DR4

    Range: 31.25 pg/ml - 1000 pg/ml

    Sensitivity < 8 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceHuman Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Alternative names
        APO2 CD261 Death receptor 4
        TNFRSF10ATRAIL receptor 1TRAILR1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46510 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      DR4 Human ELISA Kit images

      • ELISA - Human DR4 ELISA Kit (ab46510)
        ELISA - Human DR4 ELISA Kit (ab46510)
        Typical DR4 standard curve ranging from 31.25 to 1000 pg/mL.

      References for DR4 Human ELISA Kit (ab46510)

      ab46510 has not yet been referenced specifically in any publications.

      Publishing research using ab46510 ? Please let us know so that we can cite the reference in this datasheet

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      Inquiry: Dear Sir/Madam, Which lysis buffer for preparing the cell lysates does Abcan recommend to use in the human DR4 Elisa Kit (#ab46510)? Or can we use a standard lysis buffer we use in our lab (for example 0.9% Triton X-100 or a M-Per buffer)? And it...

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      Thank you for contacting us.

      A standard lysis buffer (such as RIPA buffer) can be used to prepare the cell lysates and the use protease inhibitor is indeed recommended.

      I hope this information is helpful to you. Please do not hesitate to...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"