![Western blot - DR4 antibody [32A242] (ab13890) image](#get_image6.jpg)
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Product name
Anti-DR4 antibody [32A242]
See all DR4 products (12) ...
Description
Mouse monoclonal [32A242] to DR4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Peptide corresponding to amino acid 1 to 20 of human DR4 mature protein.
Positive control
HeLa cells lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
32A242
Isotype
IgG1
Research areas
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Western blot - DR4 antibody [32A242] (ab13890)
![Western blot - DR4 antibody [32A242] (ab13890) image](/ps/datasheet/Images/13/ab13890/get_image6.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 52 kDa).
Target
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Tissue specificity
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionO00220
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apo 2 antibody
- Apo2 antibody
- CD 261 antibody
see all
Database links
- Entrez Gene: 8797 Human
- Omim: 603611 Human
- SwissProt: O00220 Human
- Unigene: 213467 Human
- Unigene: 591834 Human
Anti-DR4 antibody [32A242] images:
Western blot - DR4 antibody [32A242] (ab13890)
Predicted band size : 52 kDa
Western blot analysis of 20 µg of total cell lysate from HeLa cells with ab13890 at 2 ug/ml dilution.
References for Anti-DR4 antibody [32A242] (ab13890)
This product has been referenced in:
- Choi H et al. Peroxiredoxin 6 interferes with TRAIL-induced death-inducing signaling complex formation by binding to death effector domain caspase. Cell Death Differ 18:405-14 (2011). WB; Human.Read more (PubMed: 20829884) »
- Fan S et al. Scatter factor protects tumor cells against apoptosis caused by TRAIL. Anticancer Drugs 21:10-24 (2010).Read more (PubMed: 19823077) »
See all 4 publications for this product
Publishing research using ab13890? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"