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Product name
Anti-DR4 antibody
See all DR4 products (12) ...
Description
Rabbit polyclonal to DR4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ASGTEAAAATPSKVWGSSAG, corresponding to amino acids 24/43 of Human DR4 (Peptide available as ab8447 .)
ASGTEAAAATPSKVWGSSAG
Positive control
HeLa or Jurkatwhole cell lysate A 57 kDa band should be detected.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Western blot - DR4 antibody (ab8415)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with DR4 peptide (24/43) (ab8447).
Target
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Tissue specificity
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionO00220
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apo 2 antibody
- Apo2 antibody
- CD 261 antibody
see all
Database links
- Entrez Gene: 8797 Human
- Omim: 603611 Human
- SwissProt: O00220 Human
- Unigene: 213467 Human
- Unigene: 591834 Human
Anti-DR4 antibody images:
Western blot - DR4 antibody (ab8415)
All lanes : Anti-DR4 antibody (ab8415)
Lane 1 : H: HeLa
Lane 2 : K: K562
Lane 3 : J: Jurkat whole cell lysate
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Western blot analysis of DR4 at 1:500 dilution.
References for Anti-DR4 antibody (ab8415)
This product has been referenced in:
- Köhler B et al. Bid participates in genotoxic drug-induced apoptosis of HeLa cells and is essential for death receptor ligands' apoptotic and synergistic effects. PLoS ONE 3:e2844 (2008). WB; Human.Read more (PubMed: 18665234) »
See 1 publication for this product
Publishing research using ab8415? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"