DR5 Human ELISA Kit (ab46014)

Overview

• Product nameDR5 Human ELISA KitSee all DR5 kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Sample A	8	483pg/ml	7	1.39%
  Sample B	8	117pg/ml	4	3.23%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Sample A	9	679pg/ml	45	6.58%
  Sample B	9	186pg/ml	14	7.44%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Cell culture extracts
• Assay typeSandwich
• Sensitivity
  < 6 pg/ml
• Range
  31.25 pg/ml - 1000 pg/ml
• Assay time
  2h 30m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The Human DR5 ELISA is to be used for the in-vitro quantitative determination of DR5 in human serum, buffered solutions, cell culture medium or cell lysate. The assay will recognize both natural and recombinant human DR5. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The DR5 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for DR5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR5 concentrations and unknowns are pipetted into these wells.

  During the first incubation, the DR5 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for DR5 is incubated. Then the enzyme (streptavidin-horse radish peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of DR5 present in the samples.

   

  Recognizes Human DR5
  
  Range: 31.25 pg/ml - 1000 pg/ml
  
  Sensitivity < 6 pg/ml
  

• Notes

  Safety Information:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  10X Standard Diluent Buffer	Black	1 x 25ml
  200X Washing Buffer	White	2 x 10ml
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Biotinylated anti-Trail R2/DR5 (CD262)	Red	2 x 0.4ml
  Chromogen TMB		1 x 24ml
  DR5 precoated microtitre plates		8 units
  DR5 Standard	Yellow	4 vials
  HRP Diluent	Red	1 x 23ml
  Stop Reagent	Black	2 x 11ml
  Streptavidin HRP		4 x 5µl

• Research Areas
  • Cancer
  • Growth factors
  • TNF

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Apoptosis marker and proteins ELISA kits

  • Signal Transduction
  • Growth Factors/Hormones
  • TNF

  • Cell Biology
  • Apoptosis
  • Receptors
  • Death Receptors

Applications

DR5 Human ELISA Kit images

• 

  ELISA - Human DR5 ELISA kit (ab46014)
  Typical DR5 standard curve ranging from 1000 to 31.25 pg/mL

Protocols

• Human DR5 ELISA kit