Overview
Key features of DRAQ5™ include:
- Convenient ready-to-use aqueous solution
- Rapid uptake into living cells, providing a high level of nuclear discrimination
- No photobleaching effect
- It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.
- No compensation needed with common FITC/GFP + PE combinations in flow cytometry
- No RNase treatment required
SPECTRAL PROPERTIES:
Excitation
- 647 nm line optimal (Exmax 646 nm)
- 488, 514, 568 and 633 nm lines, sub-optimal
- Two-photon excitation (1047 nm) and excitation dark (700-850 nm)
Emission (instrument dependent):
- 665 nm to infra-red <800 nm (Emmax 681 nm / 697 nm intercalated with dsDNA)
- Minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695L, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
- No photobleaching effect
- No fluorescence enhancement upon DNA binding
- Compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes
Properties
Concentration information loading...Applications
Our Abpromise guarantee covers the use of ab108410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Fluorescence Microscopy | Fluorescence Microscopy: Use at an assay dependent concentration. |
| Flow Cyt | Flow Cyt: 1/1000 - 1/250. For Gating of Nucleated cells = 5µM; For Cell Cycle Analysis = 20µM |
| ICC/IF | ICC/IF: 1/1000. For Cell-based assays, Immunofluorescence microscopy and In-Cell WB = 5µM It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet section. |
DRAQ5™ 50 µl (5mM) images
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Immunocytochemistry/ Immunofluorescence - DRAQ5™ 50 µl (5mM) (ab108410)HeLa cells were stained with Lamin B1 antibody - Nuclear Envelope Marker (ab16048) and alpha Tubulin antibody [DM1A] - Loading Control (ab7291). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibodies (ab16048 & ab7291) at 1µg/ml overnight at 4C.. The secondary antibodies were Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899) (green) and Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed (ab96881) (red) used at 1/250 dilution for 1h at room temperature. 5µM DRAQ5 was added to the secondary antibody mixture to label nuclear DNA (pseudocolor purple). -
Immunocytochemistry/ Immunofluorescence - DRAQ5™ 50 µl (5mM) (ab108410)HeLa cells were stained with beta Catenin antibody (ab16051) and alpha Tubulin antibody [DM1A] - Loading Control (ab7291). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibodies (ab16051 & ab7921) at 1µg/ml overnight at 4C. The secondary antibodies were Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899) (green) and Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (ab96899) (red) used at 1/250 dilution for 1h at room temperature. 5µM DRAQ5 was added to the secondary antibody mixture to label nuclear DNA (pseudocolor orange). -
Immunocytochemistry/ Immunofluorescence - DRAQ5™ 50 µl (5mM) (ab108410)DRAQ5™-stained nuclei in a adult Drosophila brain.
Protocols
References for DRAQ5™ 50 µl (5mM) (ab108410)
This product has been referenced in:
- Daigle JG et al. RNA-binding ability of FUS regulates neurodegeneration, cytoplasmic mislocalization and incorporation into stress granules associated with FUS carrying ALS-linked mutations. Hum Mol Genet 22:1193-205 (2013). Read more (PubMed: 23257289) »
- Keller KE et al. Inhibition of hyaluronan synthesis reduces versican and fibronectin levels in trabecular meshwork cells. PLoS One 7:e48523 (2012). ICC/IF . Read more (PubMed: 23139787) »
