RabMAb

Anti-DUT antibody [EPR9608(B)] (ab137097)

Overview

  • Product nameAnti-DUT antibody [EPR9608(B)]See all DUT primary antibodies ...
  • Description
    Rabbit monoclonal [EPR9608(B)] to DUT
  • Tested applicationsICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, 293T, Ramos and HepG2 cell lysates; HepG2 cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab137097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IP 1/100 - 1/500.
Flow Cyt 1/100 - 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificityFound in a variety of tissues.
    • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similaritiesBelongs to the dUTPase family.
    • Post-translational
      modifications
      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody [EPR9608(B)] images

    • All lanes : Anti-DUT antibody [EPR9608(B)] (ab137097) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 26, 18 kDa
    • Immunofluorescent analysis of HepG2 cells labelling DUT with ab137097 at 1/250 dilution.

    References for Anti-DUT antibody [EPR9608(B)] (ab137097)

    ab137097 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"