RabMAb

Anti-DUT antibody [EPR9608(B)] (ab137097)

Overview

  • Product nameAnti-DUT antibody [EPR9608(B)]
    See all DUT primary antibodies
  • Description
    Rabbit monoclonal [EPR9608(B)] to DUT
  • Tested applicationsICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, 293T, Ramos and HepG2 cell lysates; HepG2 cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab137097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IP 1/100 - 1/500.
Flow Cyt 1/100 - 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificityFound in a variety of tissues.
    • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similaritiesBelongs to the dUTPase family.
    • Post-translational
      modifications
      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody [EPR9608(B)] images

    • All lanes : Anti-DUT antibody [EPR9608(B)] (ab137097) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 26, 18 kDa
    • Immunofluorescent analysis of HepG2 cells labelling DUT with ab137097 at 1/250 dilution.

    References for Anti-DUT antibody [EPR9608(B)] (ab137097)

    ab137097 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"