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Rabbit monoclonal [EPR9608(B)] to DUT
WB more details Unsuitable for:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
HeLa, 293T, Ramos and HepG2 cell lysates; HepG2 cells.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/100 - 1/500.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.
Is unsuitable for IHC-P.
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Found in a variety of tissues.
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Belongs to the dUTPase family.
Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Mitochondrion and Nucleus.
Information by UniProt
Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
Deoxyuridine triphosphatase antibody
Western blot - Anti-DUT antibody [EPR9608(B)] (ab137097)
All lanes : Anti-DUT antibody [EPR9608(B)] (ab137097) at 1/10000 dilution Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : Ramos cell lysate Lane 4 : HepG2 cell lysate Lysates/proteins at 10 µg per lane. Secondary HRP-labelled Goat anti-Rabbit at 1/2000 dilution Predicted band size : 26, 18 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DUT antibody [EPR9608(B)] (ab137097)
Immunofluorescent analysis of HepG2 cells labelling DUT with ab137097 at 1/250 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"