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Can you tell me if these products, ab16140, ab65220, ab69811, ab71464 will only detect 「Dyrk1A」? It seems that similar products from other companies will also detect 「DYRK1B」 and 「 DYRK2」. |
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ANSWER: |
Thank you for your enquiry. ab16140：The antigen made to generate the antibody is specific to Dyrk1A. That said, we have never actually tested against any other Dyrk protein, but the Dyrk1A sequence differs so greatly from Dyrk1B that it would be highly unlikely for the antibody to cross react. ab65220：The antibody only can detect DYRK1A according to the BLAST result. ab71464：There is no information on cross reactivity with 「DYRK1B」or「 DYRK2」. ab69811: Cross reactivity with DYRK1B and DYRK2 has not been tested. However using the immunising sequence suggested in the datasheet (733-752 a.a) in a BLAST search, it appears there is little homology between the immunising sequence and DYRK1B and DYRK2. I hope this information will be helpful. |
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Is there an anti-phospho specific antibody for DYRK1A?
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ANSWER: |
Thank you for your enquiry. We do not have an anti-phospho specific antibody for DYRK1A at this time, but we gladly accept all suggestions and may decide to pursue this opportunity in the future. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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We were looking for a antibodies that recognise Drosophila Dyrk1A/minibrain. We found that you sell a DYRK1A antibody. We wondered whether you could tell us whether the peptide antigen used to generate this antibody has homology with the Drosophila protein (below), so that we can decide whether or not to purchase it. Also, we wanted to know if you have any antibody that bind the activation loop of DYRK1A or any of the related kinases (in the CMGC group), which we could test or use to help use design a such a phosphospecific antibody to this protein? Attached is the sequence MHHHSSPSSSSEVRAMQARIPNHFREPASGPLRKLSVDLIKTYKHINEVYYAKKKRRAQQTQGDDDSSNKKERKLYNDGYDDDNHDYIIKNGEKFLDRYEIDSLIGKGSFGQVVKAYDHEEQCHVAIKIIKNKKPFLNQAQIEVKLLEMMNRADAENKYYIVKLKRHFMWRNHLCLVFELLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLSTPELNIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYHYIQSRFYRSPEVLLGIQYDLAIDMWSLGCILVEMHTGEPLFSGCNEVDQMNKIVEVLGMPPKYLLDQAHKTRKFFDKIVADGSYVLKKNQNGRKYKPPGSRKLHDILGVETGGPGGRRLDEPGHSVSDYLKFKDLILRMLDFDPKTRVTPYYALQHNFFKRTADEATNTSGAGATANAGAGGSGSSGAGGSSGGGVGGGLGASNSSSGAVSSSSAAAPTAATAAATAAGSSGSGSSVGGGSSAAQQQQAMPLPLPLPLPLPPLAGPGGASDGQCHGLLMHSVAANAMNNFSALSLQSNAHPPPSLANSHHSTNSLGSLNHISPGSTGCHNNNSNSSNNNTRHSRLYGSNMVNMVGHHNSGSSNNHNSISYPHAMECDPPQMPPPPPNGHGRMRVPAIMQLQPNSYAPNSVPYYGNMSSSSVAAAAAAAAAAASHLMMTDSSVISASAAGGGQGGGNPGQNPVTPSAAAFLFPSQPAGTLYGTALGSLSDLPLPMPLPMSVPLQLPPSSSSSVSSGSASVGSGGVGVGVVGQRRHITGPAAQVGISQSVGSGSSGSATGASSSDASSSSPMVGVCVQQNPVVIH Thank you |
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ANSWER: |
Thank you for your enquiry. The peptide used to produce this antibody was a recombinant fragment corresponding to amino acids 486-763 of Human DYRK1A. I did an alignment of this fragment of DYRK1A with the Drosophila sequence you provided to me, and it appears that this antibody would not be useful for you. There is not a good degree of homology between the sequences, so I don't believe this antibody will recognize the drosophila protein very well. Here are the reulsts of the alignment (I hope it shows up correctly in this email): SeqA Name Len(aa) SeqB Name Len(aa) Score =========================================================== 1 drosophila 843 2 immunogen 283 21 =========================================================== CLUSTAL W (1.82) multiple sequence alignment drosophila MHHHSSPSSSSEVRAMQARIPNHFREPASGPLRKLSVDLIKTYKHINEVYYAKKKRRAQQ 60 immunogen ------------------------------------------------------------ ::.::::. : .: .. ..::.. . : . .: . .. :... :.. drosophila TQGDDDSSNKKERKLYNDGYDDDNHDYIIKNGEKFLDRYEIDSLIGKGSFGQVVKAYDHE 120 immunogen ------------------------------------------------------------ :.....::.... . ... .... . ..... . . .: ...: .. .: . . drosophila EQCHVAIKIIKNKKPFLNQAQIEVKLLEMMNRADAENKYYIVKLKRHFMWRNHLCLVFEL 180 immunogen ------------------------------------------------------------ ... : . ..... ..:. . . . . :.:... . . . . . drosophila LSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLSTPELNIIHCDLKPENILLCNPKR 240 immunogen ------------------------------------------------------------ : . . .:. . : . : . :.. .:: ::.. . .. .... .... drosophila SAIKIVDFGSSCQLGQRIYHYIQSRFYRSPEVLLGIQYDLAIDMWSLGCILVEMHTGEPL 300 immunogen ------------------------------------------------------------ :: . . .::.. .. .: :.. . . . : . : .. . :... drosophila FSGCNEVDQMNKIVEVLGMPPKYLLDQAHKTRKFFDKIVADGSYVLKKNQNGRKYKPPGS 360 immunogen ------------------------------------------------------------ :.... .. .. . . ... ..: .: . .. :..: ...... . ....: drosophila RKLHDILGVETGGPGGRRLDEPGHSVSDYLKFKDLILRMLDFDPKTRVTPYYALQHNFFK 420 immunogen ------------------------------------------------------------ . . . .:..... .... : :. . .. . ...: :. : . . . drosophila RTADEATNTSGAGATANAGAGGSGSSGAGGSSGGGVGGGLGASNSSSGAVSSSSAAAPTA 480 immunogen KTADEGTNTS--------------------------------NSVSTSPAMEQSQSSGTT 28 :****.****.:.:::.:.:..:.::.:..::... ... .:.. *:... ..* :: *: drosophila ATAAATAAGSSGSGSSVGGGSSAAQQQQAMPLPLPLPLPLPPLAGPGGASDGQCHGLLMH 540 immunogen SSTSSSSGGSSGTSNSGRARSDPTHQHRHS----------------GGHFTAAVQAMDCE 72 :::::::.****:..* . *..::*:: . . . . . .. :..** . :.: . drosophila SVAANAMNNFSALSLQSNAHPPPSLANSHHSTNSLGSLNHISPGSTGCHNNNSNSSNNNT 600 immunogen THSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQET--TFHVAPQQNALHHHHGNSSHHHH 130 : :.:. ::*.* *.:..*..:: . *..:. .: *::* ... *:::.***::: drosophila RHSRLYGSNMVNMVGHHNSGSSNNHNSISYPHAMECDPPQMPPPPPNGHGRMRVPAIMQL 660 immunogen HHHHHHHHHGQQALGNRTR-------------------PRVYNSPTN------------- 158 :* : : : : :*::. .::.. .: : . : ....*:: .*.*. . .: . drosophila QPNSYAPNSVPYYGNMSSSSVAAAAAAAAAAASHLMMTDSSVISASAAGGGQGGGNPGQN 720 immunogen ---------------SSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNR 203 ...: :..: . .. **:. : .. : : : * : :* ::*:: *.**. :. drosophila PVTPSAAAFLFPSQPAGTLYGTALGSLSDLPLPMPLPMSVPLQLPPSSSSSVSSGSASVG 780 immunogen PVAANTLDFGQNG-----------------------AMDVNLTVYSNPRQ---------- 230 **:..: * ...:.: .:: .: :. . . . .*.* * : ... .: ::.::: . drosophila SGGVGVGVVGQRRHITGPAAQVGISQSVGSGSSGSATGASSSDASSSSPMVGVCVQQNPV 840 immunogen ----ETGIAGHPTYQFS--ANTGPAHYMTEGHLTMRQGADREES----PMTGVCVQQSPV 280 :.. .*:.*: : ..:*:.* :: : .* **. .::::::**.******.** drosophila VIH 843 immunogen ASS 283 Unfortunately this is the only DYRK1A antibody we sell. I would recommend doing a search on our site for the other kinases in the same group, as I do not have any expertise in this area and do not know what to search for. You may also wish to use our "World's Antibody Gateway" link at the top of every search results page to look for additional antibodies that may be available through 245 other companies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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