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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-DYRK1A antibody
See all DYRK1A products (6) ...
Description
Mouse monoclonal to DYRK1A
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: NQGNQAYQNR PVAANTLDFG QNGAMDVNLT VYSNPRQETG IAGHPTYQFS ANTGPAHYMT EGHLTMRQGA DREESPMTGV CVQQSPVASS , corresponding to amino acids 674-763 of Human DYRK1A
NQGNQAYQNR PVAANTLDFG QNGAMDVNLT VYSNPRQETG IAGHPTYQFS ANTGPAHYMT EGHLTMRQGA DREESPMTGV CVQQSPVASS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 86 kDa.
IP: Use at an assay dependent dilution. (PubMed: 20053746)
Flow Cyt: Use 1µg for 106 cells.
Target
Relevance
DYRK1A, the vertebrate of Drosophilia Minibrain, is a dual-specificity kinase predominately expressed in the central nervous system. The human clone has been isolated from the Downs' syndrome critical region and it is potentially implicated in the neuropathology of the disease. Main features of the protein include an N-terminal nuclear translocation signal, a putative leucine zipper domain, a core kinase domain with some similarity to kinases involved in cell cycle regulation and a C-terminal PEST sequence. The DYRK1A kinase can be phosphorylated on tyrosine residues, leading to an active kinase that can phosphorylated itself or exogenous substrates on both tyrosine and serine/threonine residues. DYRK1A can also multimerize and translocate to the nucleus. Present studies on DYRK1A suggest a potential role for this kinase in the exit from the cell cycle and the beginning of neuronal differentiation.
Cellular localization
Nuclear
Alternative names
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1A antibody
- Dual specificity YAK 1 related kinase antibody
see all
Database links
- Entrez Gene: 1859 Human
- Omim: 600855 Human
- SwissProt: Q13627 Human
- Unigene: 368240 Human
Anti-DYRK1A antibody images:
Western blot - DYRK1A antibody (ab54944)
DYRK1A antibody (ab54944) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-DYRK1A antibody (ab54944)
Overlay histogram showing HeLa cells stained with ab54944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54944, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-DYRK1A antibody (ab54944)
This product has been referenced in:
- Komorek Jet al. Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human papillomaviruses suppress cell transformation through interaction with FOXK1/K2 transcription factors. J Virol 84:2719-31 (2010). WB, IP; Human.Read more (PubMed: 20053746) »
See 1 publication for this product
Publishing research using ab54944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"