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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-DYRK1A antibody
See all DYRK1A products (6) ...
Description
Rabbit polyclonal to DYRK1A
Specificity
ab65220 detects endogenous levels of total DYRK1A protein.
Tested applications
ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from an internal sequence of human DYRK1A
Positive control
WB: HepG2 cell extracts. ICC/IF: HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65220 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000.Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Target
Relevance
DYRK1A, the vertebrate of Drosophilia Minibrain, is a dual-specificity kinase predominately expressed in the central nervous system. The human clone has been isolated from the Downs' syndrome critical region and it is potentially implicated in the neuropathology of the disease. Main features of the protein include an N-terminal nuclear translocation signal, a putative leucine zipper domain, a core kinase domain with some similarity to kinases involved in cell cycle regulation and a C-terminal PEST sequence. The DYRK1A kinase can be phosphorylated on tyrosine residues, leading to an active kinase that can phosphorylated itself or exogenous substrates on both tyrosine and serine/threonine residues. DYRK1A can also multimerize and translocate to the nucleus. Present studies on DYRK1A suggest a potential role for this kinase in the exit from the cell cycle and the beginning of neuronal differentiation.
Cellular localization
Nuclear
Alternative names
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1A antibody
- Dual specificity YAK 1 related kinase antibody
see all
Database links
- Entrez Gene: 1859 Human
- Entrez Gene: 13548 Mouse
- Entrez Gene: 25255 Rat
- Omim: 600855 Human
- SwissProt: Q13627 Human
- SwissProt: Q61214 Mouse
- SwissProt: Q63470 Rat
- Unigene: 368240 Human
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Anti-DYRK1A antibody images:
Immunocytochemistry/ Immunofluorescence - DYRK1A antibody (ab65220)
ab65220, at a 1/500 dilution, staining DYRK1A in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - DYRK1A antibody (ab65220)
All lanes : Anti-DYRK1A antibody (ab65220) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 86 kDa
Observed band size : 86 kDa
References for Anti-DYRK1A antibody (ab65220)
This product has been referenced in:
- Baek KHet al. Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1. Nature 459:1126-30 (2009). WB; Human.Read more (PubMed: 19458618) »
See 1 publication for this product
Publishing research using ab65220? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"