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All lanes : Anti-DZIP1 antibody (ab68568) at 1/500 dilution
Lane 1 : DZIP1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 99 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
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