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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Dab1 (phospho S491) antibody
See all Dab1 products (7) ...
Description
Rabbit polyclonal to Dab1 (phospho S491)
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken
Immunogen
Synthetic phosphopeptide derived from the region of human Dab1 that contains serine 491.
Positive control
Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - Anti-Dab1 (phospho S491) antibody (ab5776)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Detects a band of approximately 80 kDa.
When examining phosphorylation of endogenous Dab1 protein we recommend that Dab1 protein first be immunoprecipitated.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Adapter molecule functioning in neural development. May regulate SIAH1 activity.
Sequence similarities
Contains 1 PID domain.
Domain
The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
Post-translational
modifications
Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
Target information above from: UniProt accessionO75553
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dab 1 antibody
- Dab1 antibody
- DAB1_HUMAN antibody
see all
Database links
- Entrez Gene: 374083 Chicken
- Entrez Gene: 1600 Human
- Entrez Gene: 13131 Mouse
- Entrez Gene: 266729 Rat
- Omim: 603448 Human
- SwissProt: Q6XBN6 Chicken
- SwissProt: O75553 Human
- SwissProt: P97318 Mouse
see all
Anti-Dab1 (phospho S491) antibody images:
Western blot - Anti-Dab1 (phospho S491) antibody (ab5776)
Mutant Analysis: Lysates prepared from HEK293 cells transfected with wild type (1, 2) or S491A mutant mouse Dab1 (3, 4) and left untreated (-) (1, 3) or treated with H2O2 (+) (2, 4) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with ab5776 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show H2O2 stimulation of the phospho-Dab1 signal only in wild type Dab1-expressing cells. These results demonstrate the specificity of ab5776 antibody for this site on the Dab1 protein.
References for Anti-Dab1 (phospho S491) antibody (ab5776)
ab5776 has not yet been referenced specifically in any publications.
Publishing research using ab5776? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"