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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab5673? Please let us know so that we can cite the reference in this datasheet
ab5673 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-Dab1 (phospho Y198) antibody (ab5673)
Mutant Analysis: Lysates prepared from HEK293 cells transfected with wild type (1, 2) or Y198F mutant mouse Dab1 (3, 4) and left untreated (-) (1, 3) or treated with H2O2 (+) (2, 4) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with ab5673 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show H2O2 stimulation of the phospho-Dab1 signal only in wild type Dab1-expressing cells. These results demonstrate the specificity of the ab5673 antibody for this site on the Dab1 protein.
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