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- Dab1 (phospho Y198) antibody (ab5673)
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Product Name
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Dab1 (phospho Y198) antibody
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See all Dab1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dab1 (phospho Y198)
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Immunogen
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Synthetic phosphopeptide derived from the region of human Dab1 that contains tyrosine 198.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Chk
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 80 kDa.
When examining phosphorylation of endogenous Dab1 protein we recommend that Dab1 protein first be immunoprecipitated.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein.
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Neuroscience >> Neurology process >> Neural Signal Transduction
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Relevance
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Disruption of DAB 1 (Disable 1) results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. DAB1 acts downstream of reelin in a signalling pathway.
Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled 1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 tyrosine 198 is a major site for reelin-induced Src family kinase-mediated phosphorylation in embryonic neurons.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dab1 (phospho Y198) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dab1 (phospho Y198) antibody images, references, products related to ab5673 and other tools.
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Dab1 (phospho Y198) antibody images:
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 - Dab1 (phospho Y198) antibody (ab5673)
Mutant Analysis: Lysates prepared from HEK293 cells transfected with wild type (1, 2) or Y198F mutant mouse Dab1 (3, 4) and left untreated (-) (1, 3) or treated with H2O2 (+) (2, 4) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with ab5673 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show H2O2 stimulation of the phospho-Dab1 signal only in wild type Dab1-expressing cells. These results demonstrate the specificity of the ab5673 antibody for this site on the Dab1 protein.
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Search PubMed (MEDLINE) for references to Dab1
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