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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab42380)
ICC/IF image of ab42380 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42380, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Dact1 / Dapper homolog 1 antibody (ab42380)
All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab42380) at 1 µg/ml
Lane 1 : Mouse Embryonic Germ Cell Lysate (ab52409)
Lane 2 :
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 84 kDa
Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
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