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Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab42380)
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Western blot - Dact1 / Dapper homolog 1 antibody (ab42380)
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Product Name
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Dact1 / Dapper homolog 1 antibody
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See all Dact1 / Dapper homolog 1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dact1 / Dapper homolog 1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Mouse Dact1 / Dapper homolog 1. (Peptide available as ab42379.)
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Mouse Brain tissue lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Members of the Dact protein family were initially identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signaling. Dact1 inhibits Wnt signaling. During mouse development, Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues and in the central nervous system (CNS). Dact paralogs have distinct signaling and developmental roles that suggest they may differentially contribute to postnatal brain physiology.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 59036 (Mouse)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact1 / Dapper homolog 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dact1 / Dapper homolog 1 antibody images, references, products related to ab42380 and other tools.
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Dact1 / Dapper homolog 1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab42380)
ICC/IF image of ab42380 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42380, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Western blot - Dact1 / Dapper homolog 1 antibody (ab42380)
All lanes : Dact1 / Dapper homolog 1 antibody (ab42380) at 1 µg/ml
Lane 1 : Mouse Embryonic Germ Cell Lysate (ab52409)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 84 kDa
Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Dact1 / Dapper homolog 1
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Dact1 / Dapper homolog 1 antibody - more information
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