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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Dact1 / Dapper homolog 1 antibody
See all Dact1 / Dapper homolog 1 products (5) ...
Description
Rabbit polyclonal to Dact1 / Dapper homolog 1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Mouse Dact1 / Dapper homolog 1.
(Peptide available as ab42379.)
Positive control
This antibody gave a positive signal in Mouse Brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
Target
Function
Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ9NYF0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dact antibody
- Dact1 antibody
- DACT1_HUMAN antibody
see all
Database links
- Entrez Gene: 59036 Mouse
- SwissProt: Q8R4A3 Mouse
- Unigene: 46662 Mouse
Anti-Dact1 / Dapper homolog 1 antibody images:
Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab42380)
ICC/IF image of ab42380 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42380, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Dact1 / Dapper homolog 1 antibody (ab42380)
All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab42380) at 1 µg/ml
Lane 1 : Mouse Embryonic Germ Cell Lysate (ab52409)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 84 kDa
Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Dact1 / Dapper homolog 1 antibody (ab42380)
ab42380 has not yet been referenced specifically in any publications.
Publishing research using ab42380? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"