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Western blot - Dact1 / Dapper homolog 1 antibody (ab42547)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dact1 / Dapper homolog 1 antibody (ab42547)
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Product Name
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Dact1 / Dapper homolog 1 antibody
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See all Dact1 / Dapper homolog 1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dact1 / Dapper homolog 1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human Dact1 / Dapper homolog 1. (Peptide available as ab42546.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a doublet band of approximately 90 and 98 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Jurkat and A431 whole cell lysates.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Members of the Dact protein family were initially identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signaling. Dact1 inhibits Wnt signaling. During mouse development, Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues and in the central nervous system (CNS). Dact paralogs have distinct signaling and developmental roles that suggest they may differentially contribute to postnatal brain physiology.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.700 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact1 / Dapper homolog 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dact1 / Dapper homolog 1 antibody images, references, products related to ab42547 and other tools.
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Dact1 / Dapper homolog 1 antibody images:
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 Western blot - Dact1 / Dapper homolog 1 antibody (ab42547)
All lanes : Dact1 / Dapper homolog 1 antibody (ab42547) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 90 kDa
Observed band size : 90,98 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dact1 / Dapper homolog 1 antibody (ab42547)
IHC image of Dact1/Dapper homolog 1 staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42547, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Search PubMed (MEDLINE) for references to Dact1 / Dapper homolog 1
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Dact1 / Dapper homolog 1 antibody - more information
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Customer reviews (feedback) regarding Dact1 / Dapper homolog 1 antibody |
Customer FAQs regarding Dact1 / Dapper homolog 1 antibody |
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Price and availability of products related to Dact1 / Dapper homolog 1 antibody |
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Dact1 / Dapper homolog 1 antibody - related products:
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Dact1 / Dapper homolog 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dact1 / Dapper homolog 1 antibody |
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