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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Dact1 / Dapper homolog 1 antibody
See all Dact1 / Dapper homolog 1 products (5) ...
Description
Rabbit polyclonal to Dact1 / Dapper homolog 1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human Dact1 / Dapper homolog 1.
(Peptide available as ab42546.)
Positive control
This antibody gave a positive signal in Jurkat and A431 whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a doublet band of approximately 90 and 98 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ9NYF0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dact antibody
- Dact1 antibody
- DACT1_HUMAN antibody
see all
Database links
- Entrez Gene: 51339 Human
- Omim: 607861 Human
- SwissProt: Q9NYF0 Human
- Unigene: 48950 Human
- Unigene: 714204 Human
Anti-Dact1 / Dapper homolog 1 antibody images:
Western blot - Dact1 / Dapper homolog 1 antibody (ab42547)
All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab42547) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 90 kDa
Observed band size : 90,98 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dact1 / Dapper homolog 1 antibody (ab42547)
IHC image of Dact1/Dapper homolog 1 staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42547, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Dact1 / Dapper homolog 1 antibody (ab42547)
ab42547 has not yet been referenced specifically in any publications.
Publishing research using ab42547? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"