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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Dact1 / Dapper homolog 1 antibody
See all Dact1 / Dapper homolog 1 products (5) ...
Description
Rabbit polyclonal to Dact1 / Dapper homolog 1
Tested applications
ICC/IF, IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fusion protein, corresponding to amino acids 280-516 of Human Dact1 / Dapper homolog 1
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 - 1/200.
IP: 1/100 - 1/200.
WB: 1/1000 - 1/5000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P: Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ9NYF0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dact antibody
- Dact1 antibody
- DACT1_HUMAN antibody
see all
Database links
- Entrez Gene: 51339 Human
- Entrez Gene: 59036 Mouse
- Omim: 607861 Human
- SwissProt: Q9NYF0 Human
- SwissProt: Q8R4A3 Mouse
- Unigene: 48950 Human
- Unigene: 714204 Human
- Unigene: 46662 Mouse
Anti-Dact1 / Dapper homolog 1 antibody images:
Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab51260)
HeLa cells stained with ab51260 Dact1/Dapper homolog 1 antibody (red) at a dilution of 1/200.
Immunoprecipitation - Dact1 / Dapper homolog 1 antibody (ab51260)
.... cell extracts immunoprecipitated with ab51260 at 1/200 dilution (lane 2) or a negative control antibody (lane 1). Immunoprecipitates were blotted with ab51260 at 1/1000 dilution.
Western blot - Dact1 / Dapper homolog 1 antibody (ab51260)
All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab51260) at 1/1000 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Molecular weight marker
Lane 3 : HEK293 transfected with Myc-Dact1 cell lysate
Predicted band size : 90 kDa
Observed band size : 90 kDa
Additional bands seen are thought to be degraded products of Dact1
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Dact1 / Dapper homolog 1 antibody(ab51260)
IHC image of ab51260 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51260, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Dact1 / Dapper homolog 1 antibody (ab51260)
ab51260 has not yet been referenced specifically in any publications.
Publishing research using ab51260? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"