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Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab51260)
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Immunoprecipitation - Dact1 / Dapper homolog 1 antibody (ab51260)
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Western blot - Dact1 / Dapper homolog 1 antibody (ab51260)
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Product Name
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Dact1 / Dapper homolog 1 antibody
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See all Dact1 / Dapper homolog 1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dact1 / Dapper homolog 1
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Immunogen
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Recombinant fusion protein, corresponding to amino acids 280-516 of Human Dact1 / Dapper homolog 1
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Reacts with
(species key)
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Hu, Ms
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100 - 1/200.
IP: 1/100 - 1/200.
WB: 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Members of the Dact protein family were initially identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signaling. Dact1 inhibits Wnt signaling. During mouse development, Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues and in the central nervous system (CNS). Dact paralogs have distinct signaling and developmental roles that suggest they may differentially contribute to postnatal brain physiology.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact1 / Dapper homolog 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dact1 / Dapper homolog 1 antibody images, references, products related to ab51260 and other tools.
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Dact1 / Dapper homolog 1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Dact1 / Dapper homolog 1 antibody (ab51260)
HeLa cells stained with ab51260 Dact1/Dapper homolog 1 antibody (red) at a dilution of 1/200.
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Immunoprecipitation - Dact1 / Dapper homolog 1 antibody (ab51260)
.... cell extracts immunoprecipitated with ab51260 at 1/200 dilution (lane 2) or a negative control antibody (lane 1). Immunoprecipitates were blotted with ab51260 at 1/1000 dilution.
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Western blot - Dact1 / Dapper homolog 1 antibody (ab51260)
All lanes : Dact1 / Dapper homolog 1 antibody (ab51260) at 1/1000 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Molecular weight marker
Lane 3 : HEK293 transfected with Myc-Dact1 cell lysate
Predicted band size : 90 kDa
Observed band size : 90 kDa
Additional bands seen are thought to be degraded products of Dact1
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References for Dact1 / Dapper homolog 1 antibody (ab51260)
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This product has been used in:
Zhang L et al. Dapper 1 antagonizes Wnt signaling by promoting dishevelled degradation. J Biol Chem 281:8607-12 (2006). PubMed: 16446366
If you publish research using ab51260 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Dact1 / Dapper homolog 1
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Dact1 / Dapper homolog 1 antibody - more information
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Customer reviews (feedback) regarding Dact1 / Dapper homolog 1 antibody |
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Dact1 / Dapper homolog 1 antibody - related products:
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Dact1 / Dapper homolog 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dact1 / Dapper homolog 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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