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Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
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Product Name 

Dact1 / Dapper homolog 1 antibody

 

See all Dact1 / Dapper homolog 1 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Dact1 / Dapper homolog 1

Immunogen 

A portion of amino acids 725-775 of human Dact1 / Dapper homolog 1, (NP_057735).

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Chk, Chmp, Xl, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately 24 and 110 kDa (predicted molecular weight: 90 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human, mouse and rat brain lysate.

Cellular localization 

Cytoplasmic

Research areas 

Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

Members of the Dact protein family were initially identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signaling. Dact1 inhibits Wnt signaling. During mouse development, Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues and in the central nervous system (CNS). Dact paralogs have distinct signaling and developmental roles that suggest they may differentially contribute to postnatal brain physiology.

 

Dact1 / Dapper homolog 1 antibody (ab72078)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

452938    (Chimpanzee)
51339    (Human)
59036    (Mouse)
500666    (Rat)
405799    (Zebrafish)

Omim

    

607861    (Human)

SwissProt

    

Q9NYF0    (Human)
Q8R4A3    (Mouse)
Q8QG92    (Xenopus laevis)
Q6QZN6    (Zebrafish)

Unigene

    

48950    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact1 / Dapper homolog 1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Dact1 / Dapper homolog 1 antibody images, references, products related to ab72078 and other tools.

 

Dact1 / Dapper homolog 1 antibody images:

Product image  Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)

Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)

Lanes 1 - 2 : Dact1 / Dapper homolog 1 antibody (ab72078) at 1 µg/ml
Lanes 3 - 4 : Dact1 / Dapper homolog 1 antibody (ab72078) at 0.5 µg/ml

Lane 1 : Pre-immune sera
Lane 2 : Human brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate


Predicted band size : 90 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to Dact1 / Dapper homolog 1

 

Dact1 / Dapper homolog 1 antibody - more information

Customer reviews (feedback) regarding Dact1 / Dapper homolog 1 antibody

Customer FAQs regarding Dact1 / Dapper homolog 1 antibody

Protocols for Dact1 / Dapper homolog 1 antibody

Price and availability of products related to Dact1 / Dapper homolog 1 antibody

 

Dact1 / Dapper homolog 1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Dact1 / Dapper homolog 1 antibody

ab29449

  

Brain (Human) Cytoplasmic Lysate - adult normal tissue

ab29471

  

Brain (Rat) Cytoplasmic Lysate - normal tissue

ab30148

  

Brain (Mouse) Cytoplasmic Lysate - normal tissue

 

Compatible Secondaries for Dact1 / Dapper homolog 1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Dact1 / Dapper homolog 1 Antibodies

See all Dact1 / Dapper homolog 1 antibodies (4)...

 

Dact1 / Dapper homolog 1 antibody - other tools:

Contact Abcam with a Technical Enquiry about Dact1 / Dapper homolog 1 antibody

 

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