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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Dact1 / Dapper homolog 1 antibody
See all Dact1 / Dapper homolog 1 products (5) ...
Description
Rabbit polyclonal to Dact1 / Dapper homolog 1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Xenopus laevis, Chimpanzee, Zebrafish
Immunogen
A portion of amino acids 725-775 of human Dact1 / Dapper homolog 1, (NP_057735).
Positive control
Human, mouse and rat brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
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Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately 24 and 110 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ9NYF0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dact antibody
- Dact1 antibody
- DACT1_HUMAN antibody
see all
Database links
- Entrez Gene: 452938 Chimpanzee
- Entrez Gene: 51339 Human
- Entrez Gene: 59036 Mouse
- Entrez Gene: 500666 Rat
- Entrez Gene: 405799 Zebrafish
- Omim: 607861 Human
- SwissProt: Q9NYF0 Human
- SwissProt: Q8R4A3 Mouse
see all
Anti-Dact1 / Dapper homolog 1 antibody images:
Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
Lanes 1 - 2 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 1 µg/ml
Lanes 3 - 4 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 0.5 µg/ml
Lane 1 : Pre-immune sera
Lane 2 : Human brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size : 90 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Dact1 / Dapper homolog 1 antibody (ab72078)
ab72078 has not yet been referenced specifically in any publications.
Publishing research using ab72078? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"