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Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
(enlarge)
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Product Name
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Dact1 / Dapper homolog 1 antibody
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See all Dact1 / Dapper homolog 1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dact1 / Dapper homolog 1
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Immunogen
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A portion of amino acids 725-775 of human Dact1 / Dapper homolog 1, (NP_057735).
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Chmp, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately 24 and 110 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human, mouse and rat brain lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Members of the Dact protein family were initially identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signaling. Dact1 inhibits Wnt signaling. During mouse development, Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues and in the central nervous system (CNS). Dact paralogs have distinct signaling and developmental roles that suggest they may differentially contribute to postnatal brain physiology.
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Dact1 / Dapper homolog 1 antibody (ab72078) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact1 / Dapper homolog 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dact1 / Dapper homolog 1 antibody images, references, products related to ab72078 and other tools.
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Dact1 / Dapper homolog 1 antibody images:
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 Western blot - Dact1 / Dapper homolog 1 antibody (ab72078)
Lanes 1 - 2 : Dact1 / Dapper homolog 1 antibody (ab72078) at 1 µg/ml
Lanes 3 - 4 : Dact1 / Dapper homolog 1 antibody (ab72078) at 0.5 µg/ml
Lane 1 : Pre-immune sera
Lane 2 : Human brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size : 90 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Dact1 / Dapper homolog 1
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Dact1 / Dapper homolog 1 antibody - more information
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Customer reviews (feedback) regarding Dact1 / Dapper homolog 1 antibody |
Customer FAQs regarding Dact1 / Dapper homolog 1 antibody |
Protocols for Dact1 / Dapper homolog 1 antibody |
Price and availability of products related to Dact1 / Dapper homolog 1 antibody |
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Dact1 / Dapper homolog 1 antibody - related products:
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Dact1 / Dapper homolog 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dact1 / Dapper homolog 1 antibody |
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