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Products:Neuroscience >> Neurology process >> Neurogenesis
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Dact2 antibody
See all Dact2 products (3) ...
Description
Rabbit polyclonal to Dact2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Dact2.
(Peptide available as ab42375.)
Positive control
This antibody gave a positive signal in the following lysates: Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; Thymus (Mouse) Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Applications
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Target information above from: UniProt accessionQ5SW24
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bA503C24.7 antibody
- C6orf116 antibody
- Chromosome 6 open reading frame 116 antibody
see all
Database links
- Entrez Gene: 168002 Human
- Entrez Gene: 240025 Mouse
- Omim: 608966 Human
- SwissProt: Q5SW24 Human
- SwissProt: Q7TN08 Mouse
- Unigene: 673059 Human
- Unigene: 79993 Mouse
Anti-Dact2 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dact2 antibody (ab42099)
IHC image of Dact2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42099, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Dact2 antibody (ab42099)
All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml
Lane 1 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Western blot - Dact2 antibody (ab42099)
All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for Anti-Dact2 antibody (ab42099)
ab42099 has not yet been referenced specifically in any publications.
Publishing research using ab42099? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"