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Western blot - Dact2 antibody (ab42099)
Western blot - Dact2 antibody (ab42099)
(enlarge)


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

Product Name 

Dact2 antibody

 

See all Dact2 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Dact2

 

  Note on antibody grade

Fast-Track antibodies

  • Immunogen affinity purified
  • high ELISA titre values
  • released early (to accelerate your research)
  • 950 Abpoints to the 1st researcher
    who submits conclusive data (positive or negative):
    Read the Fast-Track antibody Terms of Use


Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Dact2.

(Peptide available as ab42375.)

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Xl
(Due to sequence homology)


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 950 Abpoints.


Read Fast Track antibody terms and conditions.


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following lysates:
Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; Thymus (Mouse) Tissue

Cellular localization 

Cytoplasmic

Research areas 

Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

Dact2 is closely related to the frd2/dpr2 sequences identified in zebrafish. Its expression is most prominent during organogenesis of the thymus, kidneys, and salivary glands, with much lower levels in the somites and in the developing CNS. It may be involved in Wnt-mediated development.

 

Alternative names Dact2 antibody (ab42099)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

168002    (Human)
240025    (Mouse)

GeneCard

    

GC06M168526    (Human)

Omim

    

608966    (Human)

SwissProt

    

Q5SW24    (Human)
Q7TN08    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.18 - 0.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Dact2 antibody images, references, products related to ab42099 and other tools.

 

Dact2 antibody images:

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'

 

Product image  Western blot - Dact2 antibody (ab42099)

Western blot - Dact2 antibody (ab42099)

All lanes : Dact2 antibody (ab42099) at 1 µg/ml

Lane 1 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 82 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)


Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

 
 

Search PubMed (MEDLINE) for references to Dact2

 

Dact2 antibody - more information

Customer reviews (feedback) regarding Dact2 antibody

Customer FAQs regarding Dact2 antibody

Protocols for Dact2 antibody

Price and availability of products related to Dact2 antibody

 

Dact2 antibody - related products:

 

Immunizing Peptide for Dact2 antibody

ab42375

  

Dact2 peptide

ab42376

  

Dact2 peptide

 

Related Products for Dact2 antibody

ab42377

  

Rabbit polyclonal to Dact2

 

Compatible Secondaries for Dact2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Dact2 Antibodies

See all Dact2 antibodies (3)...

 

Dact2 antibody - other tools:

Contact Abcam with a Technical Enquiry about Dact2 antibody

 

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