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Products:Neuroscience >> Neurology process >> Neurogenesis
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
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Related products
- Primary antibodies
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Proteins and Peptides
ab42376
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Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Publishing research using ab42377? Please let us know so that we can cite the reference in this datasheet
ab42377 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 :
Lane 4 :
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1/250 dilution
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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