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Products:Neuroscience >> Neurology process >> Neurogenesis
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
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- Primary antibodies
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Proteins and Peptides
ab42376
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Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab42377 for help.
There are no answered questions for ab42377
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 :
Lane 4 :
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1/250 dilution
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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