|
|
|
|
|
Product Name
|
Dact2 antibody
|
| |
See all Dact2 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Dact2
|
|
|
Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
|
 |
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Dact2. (Peptide available as ab42376.)
|
|
Reacts with
|
Ms
|
|
Predicted to react
(species key)
|
Xl
(Due to sequence homology)
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in the following lysates:
Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell;
This antibody also gave a positive signal in Thymus (Mouse) Tissue - data not shown
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
|
|
Relevance
|
Dact2 is closely related to the frd2/dpr2 sequences identified in zebrafish. Its expression is most prominent during organogenesis of the thymus, kidneys, and salivary glands, with much lower levels in the somites and in the developing CNS. It may be involved in Wnt-mediated development.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
|
|
|
See below for Dact2 antibody images, references, products related to ab42377 and other tools.
|
| |
Dact2 antibody images:
|
|
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
|
| |
 Western blot - Dact2 antibody (ab42377)
All lanes : Dact2 antibody (ab42377) at 1/250 dilution
Lane 1 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Dact2
|
| |
Dact2 antibody - more information
|
Customer reviews (feedback) regarding Dact2 antibody |
Customer FAQs regarding Dact2 antibody |
Protocols for Dact2 antibody |
Price and availability of products related to Dact2 antibody |
| |
Dact2 antibody - related products:
|
|
|
| |
Dact2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Dact2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
