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Products:Neuroscience >> Neurology process >> Neurogenesis
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Dact2 antibody
See all Dact2 products (3) ...
Description
Rabbit polyclonal to Dact2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Reacts with
Mouse
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Dact2.
(Peptide available as ab42376.)
Positive control
This antibody gave a positive signal in the following lysates: Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; This antibody also gave a positive signal in Thymus (Mouse) Tissue - data not shown
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Applications
Application notes
WB: 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors.
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Target information above from: UniProt accessionQ5SW24
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bA503C24.7 antibody
- C6orf116 antibody
- Chromosome 6 open reading frame 116 antibody
see all
Database links
- Entrez Gene: 240025 Mouse
- SwissProt: Q7TN08 Mouse
- Unigene: 79993 Mouse
Anti-Dact2 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Western blot - Dact2 antibody (ab42377)
All lanes : Anti-Dact2 antibody (ab42377) at 1/250 dilution
Lane 1 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Dact2 has a predicted molecular weight of 82 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam currently sell two antibodies to this target, ab42099 which detects a band at 100 kDa and ab42377 which detects a band at 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-Dact2 antibody (ab42377)
ab42377 has not yet been referenced specifically in any publications.
Publishing research using ab42377? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"