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Anti-Dact3 antibody [2A5] (ab81615)

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Overview

Product name

Anti-Dact3 antibody [2A5]
See all Dact3 products (3) ...

Description

Mouse monoclonal [2A5] to Dact3

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant fragment, corresponding to amino acids 89-202 of Human Dact3 (NP_659493)

Positive control

Mouse brain and 293T cell lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

2A5

Isotype

IgG1

Light chain type

kappa

Research areas

Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis

  • Western blot - Dact3 antibody [2A5] (ab81615)Western blot - Dact3 antibody [2A5] (ab81615) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/250 - 1/500.Detect...Read more →

    WB: 1/250 - 1/500.Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

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    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

Target

Relevance

Dact3 is the gene product of the third discovered member of the Dact (Dapper) family of developmentally-expressed genes. Discovered in mouse, a highly conserved ortholog also exists in the human genome. As with other Dact family members, Dact3 may play a role in antagonism of Wnt signaling.

Alternative names

  • Arginine rich region 1 antibody
  • Arginine rich region 1 protein antibody
  • Dapper antagonist of beta catenin 3 antibody
  • Dapper antagonist of beta catenin homolog 3 (Xenopus laevis) antibody
  • Dapper homolog 3 antibody
  • Dapper3 antibody
  • Dpr3 antibody
  • RRR1 antibody
  • Thymus expressed gene 3 like antibody
see all

Database links

Anti-Dact3 antibody [2A5] images:

  Western blot - Dact3 antibody [2A5] (ab81615)

Western blot - Dact3 antibody [2A5] (ab81615)

All lanes : Anti-Dact3 antibody [2A5] (ab81615) at 1/250 dilution

Lane 1 : mouse brain cell lysate
Lane 2 : 293T cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
goat anti-mouse HRP
developed using the ECL technique

Predicted band size : 65 kDa
Observed band size : 65 kDa

References for Anti-Dact3 antibody [2A5] (ab81615)

ab81615 has not yet been referenced specifically in any publications.

Publishing research using ab81615? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"