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Western blot - Dact3 antibody (ab42106)
Western blot - Dact3 antibody (ab42106)
(enlarge)


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

Product Name 

Dact3 antibody

 

See all Dact3 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Dact3

 

  Note on antibody grade

Fast-Track antibodies

  • Immunogen affinity purified
  • high ELISA titre values
  • released early (to accelerate your research)
  • 950 Abpoints to the 1st researcher
    who submits conclusive data (positive or negative):
    Read the Fast-Track antibody Terms of Use


Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse Dact3.

(Peptide available as ab42310.)

Predicted to react 
(species key)

Hu, Ms
(Due to sequence homology)


Abreviews 

Customer reviews
(see key)


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Read Fast Track antibody terms and conditions.


Application notes 
(see key)

Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab42310).

Not yet tested in other applications.

Research areas 

Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

Dact3 is the gene product of the third discovered member of the Dact (Dapper) family of developmentally-expressed genes. Discovered in mouse, a highly conserved ortholog also exists in the human genome. As with other Dact family members, Dact3 may play a role in antagonism of Wnt signaling.

 

Alternative names Dact3 antibody (ab42106)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

147906    (Human)
629378    (Mouse)

GeneCard

    

GC19M051844    (Human)

Omim

    

611112    (Human)

SwissProt

    

Q96B18    (Human)
Q0PHV7    (Mouse)

Unigene

    

MGC15476    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.300 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dact3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Dact3 antibody images, references, products related to ab42106 and other tools.

 

Dact3 antibody images:

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'

 

Product image  Western blot - Dact3 antibody (ab42106)

Western blot - Dact3 antibody (ab42106)

All lanes : Dact3 antibody (ab42106) at 1 µg/ml

Lane 1 : Brain (Mouse) Mouse Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 63 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)


Dact3 has a predicted molecular weight of 63 kDa; however as this is a novel protein, it may contain uncharacterised modifications that affect the migration of the protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

 
 

Search PubMed (MEDLINE) for references to Dact3

 

Dact3 antibody - more information

Customer reviews (feedback) regarding Dact3 antibody

Customer FAQs regarding Dact3 antibody

Protocols for Dact3 antibody

Price and availability of products related to Dact3 antibody

 

Dact3 antibody - related products:

 

Immunizing Peptide for Dact3 antibody

ab42310

  

Dact3 peptide

 

Compatible Secondaries for Dact3 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Dact3 Antibodies

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Dact3 antibody - other tools:

Contact Abcam with a Technical Enquiry about Dact3 antibody

 

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