Anti-Daxx antibody [E94] (ab32140)

Overview

• Product nameAnti-Daxx antibody [E94]See all Daxx primary antibodies ...
• Description
  Rabbit monoclonal [E94] to Daxx
• Tested applicationsWB, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues of human DAXX.

• Positive controlWB: Hela cell lysate. IHC-P: Human stomach adenocarcinoma.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberE94
• IsotypeIgG
• Research Areas
  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Histone chaperones

  • Cell Biology
  • Apoptosis
  • Extracellular Signals
  • Death Ligands

Applications

Target

• FunctionActs as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional corepressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the DAXX family.
• Post-translational
  modificationsSumoylated.
  Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by HIPK1 upon glucose deprivation.
  Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
• Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus > PML body. Nucleus > nucleolus. Chromosome > centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures.

Target information above from: UniProt accession Q9UER7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1616 Human
  • Omim: 603186 Human
  • SwissProt: Q9UER7 Human
  • Unigene: 336916 Human

• Alternative names
  antibodyBING 2 antibodyBING2 antibody

  CENP-C binding protein antibodyDAP 6 antibodyDAP6 antibodyDaxx antibodyDAXX antibodyDAXX_HUMAN antibodyDeath associated protein 6 antibodyDeath domain associated protein 6 antibodyDeath domain associated protein antibodyDeath domain-associated protein 6 antibodyEAP 1 antibodyEAP1 antibodyETS1 associated protein 1 antibodyETS1-associated protein 1 antibodyFas binding protein antibodyFas death domain associated protein antibodyFas death domain-associated protein antibodyhDaxx antibodyMGC126245 antibodyMGC126246 antibody

  see all

Anti-Daxx antibody [E94] images

• 

  Western blot - Daxx antibody [E94] (ab32140)
  Anti-Daxx antibody [E94] (ab32140) at 1/5000 dilution + Hela cell lysate
  
  Predicted band size : 81 kDa
  Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Paraffin-embedded sections) - Daxx antibody [E94] (ab32140)
  Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.