Anti-Daxx antibody (ab55370)

Overview

• Product nameAnti-Daxx antibodySee all Daxx primary antibodies ...
• Description
  Rabbit polyclonal to Daxx
• SpecificityDetects endogenous levels of total Daxx protein.
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic non-phosphopeptide derived from human Daxx around the phosphorylation site of Serine 668 (LPSPP)

• Positive controlExtracts from COS7 cells, treated with Serum (10% for 30 mins)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Histone chaperones

  • Cell Biology
  • Apoptosis
  • Extracellular Signals
  • Death Ligands

Applications

Target

• FunctionActs as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional corepressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the DAXX family.
• Post-translational
  modificationsSumoylated.
  Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by HIPK1 upon glucose deprivation.
  Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
• Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus > PML body. Nucleus > nucleolus. Chromosome > centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures.

Target information above from: UniProt accession Q9UER7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1616 Human
  • Entrez Gene: 13163 Mouse
  • Omim: 603186 Human
  • SwissProt: Q9UER7 Human
  • SwissProt: O35613 Mouse
  • Unigene: 336916 Human
  • Unigene: 271809 Mouse

• Alternative names
  antibodyBING 2 antibodyBING2 antibody

  CENP-C binding protein antibodyDAP 6 antibodyDAP6 antibodyDaxx antibodyDAXX antibodyDAXX_HUMAN antibodyDeath associated protein 6 antibodyDeath domain associated protein 6 antibodyDeath domain associated protein antibodyDeath domain-associated protein 6 antibodyEAP 1 antibodyEAP1 antibodyETS1 associated protein 1 antibodyETS1-associated protein 1 antibodyFas binding protein antibodyFas death domain associated protein antibodyFas death domain-associated protein antibodyhDaxx antibodyMGC126245 antibodyMGC126246 antibody

  see all

Anti-Daxx antibody images

• 

  Western blot - Daxx antibody (ab55370)
  All lanes : Anti-Daxx antibody (ab55370) at 1/500 dilution
  
  Lane 1 : extracts from COS7 cells, treated with Serum (10% for 30 mins) with no immunizing peptide
  Lane 2 : extracts from COS7 cells, treated with Serum (10% for 30 mins) with immunizing peptide
  
  
  Predicted band size : 82 kDa