Overview

  • Product nameAnti-Daxx antibody
    See all Daxx primary antibodies
  • Description
    Mouse monoclonal to Daxx
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Recombinant fragment with tag: SPVSQLFELE IEALPLDTPS SVETDISSSR KQSEEPFTTV LENGAGMVSS TSFNGGVSPH NWGDSGPPCK KSRKEKKQTG SGPLGNSYVE RQRSVHEKNG, corresponding to amino acids 561-661 of Human Daxx

  • Positive control
    • Daxx transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Daxx is approximately 1ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 82 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the DAXX family.
    • DomainThe Sumo interaction motif mediates Sumo binding, and is required both for sumoylation and binding to sumoylated targets.
    • Post-translational
      modifications
      Sumoylated with SUMO1 on multiple lysine residues.
      Phosphorylated by HIPK1 upon glucose deprivation.
      Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
    • Cellular localizationNucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383).
    • Information by UniProt
    • Database links
    • Alternative names
      • antibody
      • BING 2 antibody
      • BING2 antibody
      • CENP-C binding protein antibody
      • DAP 6 antibody
      • DAP6 antibody
      • DAXX antibody
      • DAXX_HUMAN antibody
      • Death associated protein 6 antibody
      • Death domain associated protein 6 antibody
      • Death domain associated protein antibody
      • Death domain-associated protein 6 antibody
      • EAP 1 antibody
      • EAP1 antibody
      • ETS1 associated protein 1 antibody
      • ETS1-associated protein 1 antibody
      • Fas binding protein antibody
      • Fas death domain associated protein antibody
      • Fas death domain-associated protein antibody
      • hDaxx antibody
      • MGC126245 antibody
      • MGC126246 antibody
      see all

    Anti-Daxx antibody images

    • All lanes : Anti-Daxx antibody (ab66238) at 1 µg/ml

      Lane 1 : Daxx transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 82 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    References for Anti-Daxx antibody (ab66238)

    ab66238 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (Huh7 liver)
    Specification Huh7 liver
    Permeabilization Yes - tritonx100
    Fixative Paraformaldehyde
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    Submitted Nov 24 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"