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Overview

  • Product nameDcR1 Human ELISA KitSee all DcR1 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 7171pg/ml 192 2.7%
    Sample B 8 3584pg/ml 244 6.8%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 9 7311pg/ml 515 7%
    Sample B 9 3654pg/ml 356 9.7%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    < 147 pg/ml
  • Range
    312.5 pg/ml - 10000 pg/ml
  • Assay time
    3h 40m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human DcR1 ELISA is to be used for the in-vitro quantitative determination of TNF-related apoptosis-inducing-ligand Receptor 3 (DcR1) in human serum, buffered solutions, cell culture medium or cell lysate. The assay will recognize both natural and recombinant human DcR1. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The DcR1 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations and unknowns are pipetted into these wells. During the first incubation, the DcR1 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for DcR1 is incubated. Then the enzyme ( streptavidin-horse radish peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of DcR1 present in the samples.

     

    Recognizes Human DcR1

    Range: 312.5 pg/ml - 10000 pg/ml

    Sensitivity < 147 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceDcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
  • Cellular localizationCell membrane; lipid-anchor; GPI-anchor
    • Alternative names
        Antagonist decoy receptor for TRAIL/Apo 2LCD263 CD263 antigen
        DCR1 Decoy receptor 1Decoy TRAIL receptor without death domainDR6 LIT Lymphocyte inhibitor of TRAILTNF related apoptosis inducing ligand receptor 3TRAIL R3TRAIL receptor 3Trail receptor without an intracellular domainTRAILR3TRID Tumor necrosis factor receptor superfamily member 10c decoy without an intracellular domainTumor necrosis factor receptor superfamily member 10C precursor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46016 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      DcR1 Human ELISA Kit images

      • ELISA - Human DcR1 ELISA Kit (ab46016)
        ELISA - Human DcR1 ELISA Kit (ab46016)
        Typical DcR1 standard curve ranging from 312.5 to 10000 pg/mL.

      References for DcR1 Human ELISA Kit (ab46016)

      ab46016 has not yet been referenced specifically in any publications.

      Publishing research using ab46016 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"