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Product Name
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DcR2 antibody [B-P30]
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See all DcR2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B-P30] to DcR2
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Immunogen
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Recombinant human DcR2/Fc chimera
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Reacts with
(species key)
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Hu
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Specificity
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Recognises the TRAIL R4, a 44.2 kDa protein
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use 10µl to label 106 cells or 100 µl of whole blood.
WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> TRAIL
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Cell Biology >> Apoptosis >> Receptors >> Decoy Receptors
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Relevance
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Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L, a member of the TNF family, induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are functional receptors for TRAIL, and DcR1/TRID is a decoy receptor. Another member of the TRAIL receptor family was identified and designated DcR2 (Marsters et al., 1997; Degli-Esposti et al., 1997; Pan et al., 1998). The DcR2 receptor is 386 amino acids in length and has an extracellular TRAIL binding domain, but lacks intracellular death domain and does not induce apoptosis. Although this receptor binds to the cytotoxic ligand TRAIL, it contains a truncated death domain and functions as an inhibitory receptor. When overexpressed, the DcR2 receptor can protect cells against TRAIL mediated cytotoxicity. Like DR4 and DR5, DcR2 transcript is widely expressed in a variety of normal human tissues but DcR2 is absent in most tumors. Ultraviolet radiation has been shown to upregulate DcR2 expression on human keratinocytes. Over expression of DcR2 attenuated TRAIL induced apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B-P30
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Myeloma
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x63-Ag8.653
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Isotype
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IgG1
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Purity
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Ion Exchange Chromatography
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Storage buffer
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Carrier and preservative free
Constituents: PBS
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Purification notes
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Sterile-filtered through 0.22 µm
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DcR2 antibody [B-P30] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to DcR2
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