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Western blot - Dcp1a antibody (ab47998)
(enlarge)
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Product Name
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Dcp1a antibody
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See all Dcp1a antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Dcp1a
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Immunogen
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Synthetic peptide: C-QVLTKNKDNHN, corresponding to C terminal amino acids 571-581 of Dcp1a
BLAST 'C-QVLTKNKDNHN' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: detection limit dilution 1/4000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 and HeLa Human lysates.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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Dcp1a is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. Dcp1a binds DCP1B, DCP2, RENT1 and SMAD4 and forms part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dcp1a antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dcp1a antibody images, references, products related to ab47998 and other tools.
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Dcp1a antibody images:
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 Western blot - Dcp1a antibody (ab47998)
Dcp1a antibody (ab47998) at 2 µg/ml + A431 cell lysate in RIPA buffer at 35 µg
Predicted band size : 63 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible non-specific binding).
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to Dcp1a
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Dcp1a antibody - more information
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Customer reviews (feedback) regarding Dcp1a antibody |
Customer FAQs regarding Dcp1a antibody |
Protocols for Dcp1a antibody |
Price and availability of products related to Dcp1a antibody |
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Dcp1a antibody - related products:
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Dcp1a antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dcp1a antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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