Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-Dcp2 antibody
Description
Rabbit polyclonal to Dcp2
Tested applications
ICC/IF, WB, IHC-P, EMSAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp2.
(Peptide available as ab30458.)
Positive control
This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293. This antibody gave a positive signal in the following Mouse whole cell lysate: NIH 3T3 (data not shown).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa).Can be blocked with Dcp2 peptide (ab30458).
IHC-P: 1/35Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA: Use at an assay dependent dilution. (PubMed: 20691152)
Target
Relevance
Dcp2 is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Dcp2 removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. It has higher activity towards mRNAs that lack a polyA tail. Has no activity towards a cap structure lacking a RNA moiety. Manganese is a cofactor that is required for highest activity. Dcp2 is found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, RENT1, RENT2 and RENT3B. Interacts with DCP1A, DPC1B, RENT1, RENT2 and RENT3B. The complex associates with polysomes.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- mRNA decapping enzyme 2 antibody
- Nucleoside diphosphate-linked moiety X motif 20 antibody
Database links
- Entrez Gene: 167227 Human
- SwissProt: Q8IU60 Human
- SwissProt: Q9CYC6 Mouse
Anti-Dcp2 antibody images:
Immunocytochemistry/ Immunofluorescence - Dcp2 antibody (ab28658)
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dcp2 antibody (ab28658)
Image courtesy of Human Protein Atlas
ab28658 stainning Dcp2 in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Western blot - Dcp2 antibody (ab28658)
All lanes : Anti-Dcp2 antibody (ab28658) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 48.4 kDa
Observed band size : 52,58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Dcp2 contains a number of potential phosphorylation sites (Swiss Prot data) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Dcp2 antibody (ab28658)
This product has been referenced in:
- Kim CWet al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA; Human.Read more (PubMed: 20691152) »
See 1 publication for this product
Publishing research using ab28658? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"