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Immunocytochemistry/ Immunofluorescence - Dcp2 antibody (ab28658)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dcp2 antibody (ab28658)
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Western blot - Dcp2 antibody (ab28658)
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Product Name
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Dcp2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dcp2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp2.
(Peptide available as ab30458.)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa). Can be blocked with Dcp2 peptide (ab30458).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293.
This antibody gave a positive signal in the following Mouse whole cell lysate: NIH 3T3 (data not shown).
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Relevance
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Dcp2 is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Dcp2 removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. It has higher activity towards mRNAs that lack a polyA tail. Has no activity towards a cap structure lacking a RNA moiety. Manganese is a cofactor that is required for highest activity.
Dcp2 is found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, RENT1, RENT2 and RENT3B. Interacts with DCP1A, DPC1B, RENT1, RENT2 and RENT3B. The complex associates with polysomes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.90 - 0.90 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dcp2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dcp2 antibody images, references, products related to ab28658 and other tools.
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Dcp2 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Dcp2 antibody (ab28658)
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dcp2 antibody (ab28658)
Image courtesy of Human Protein Atlas
ab28658 stainning Dcp2 in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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Western blot - Dcp2 antibody (ab28658)
All lanes : Dcp2 antibody (ab28658) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Dcp2 peptide (ab30458) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 48.4 kDa
Observed band size : 52,58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Dcp2 contains a number of potential phosphorylation sites (Swiss Prot data) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to Dcp2
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Dcp2 antibody - more information
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Customer reviews (feedback) regarding Dcp2 antibody |
Customer FAQs regarding Dcp2 antibody |
Protocols for Dcp2 antibody |
Price and availability of products related to Dcp2 antibody |
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Dcp2 antibody - related products:
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Dcp2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dcp2 antibody |
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