Dcr 2 / Dicer 2 antibody (ab4732)

Product Name 

Dcr 2 / Dicer 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Dcr 2 / Dicer 2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Fruit fly (Drosophila melanogaster) Dcr 2 / Dicer 2.

(Peptide available as ab19340.)

Reacts with 
(species key)

Dm

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 188kDa and 197kDa. Can be blocked with Dicer 2 peptide (695 - 709).

Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Dicer
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi

Relevance 

RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs.
Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.
The Saccharomyces cerevisiae cell cycle regulator genes DCR2 and DCR1 appear to be involved in the initiation of DNA replication. It has been predicted that DCR2 might encode a protein with phosphoesterase activity.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.40 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Dcr 2 / Dicer 2 antibody images, references, products related to ab4732 and other tools.

Dcr 2 / Dicer 2 antibody images:

References for Dcr 2 / Dicer 2 antibody (ab4732)

  This product has been used in:

Hartig JV et al. Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences. EMBO J 28:2932-44 (2009). WB; Fruit fly (Drosophila melanogaster). PubMed: 19644447

If you publish research using ab4732 please let us know so that we can cite the reference on this datasheet.

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