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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Dcr 2 / Dicer 2 antibody
Description
Rabbit polyclonal to Dcr 2 / Dicer 2
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Fruit fly (Drosophila melanogaster) Dcr 2 / Dicer 2.
(Peptide available as ab19340.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Dcr 2 / Dicer 2 antibody (ab4732)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Predicted molecular weight: 188kDa and 197kDa. Can be blocked with Dicer 2 peptide (695 - 709).
Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified. The Saccharomyces cerevisiae cell cycle regulator genes DCR2 and DCR1 appear to be involved in the initiation of DNA replication. It has been predicted that DCR2 might encode a protein with phosphoesterase activity.
Cellular localization
Nuclear
Alternative names
- Dcr-2 antibody
- Dcr2 antibody
- Dicer antibody
- Dicer2 antibody
Database links
- Entrez Gene: 36993 Fruit fly (Drosophila melanogaster)
- Unigene: 7764 Fruit fly (Drosophila melanogaster)
Anti-Dcr 2 / Dicer 2 antibody images:
Western blot - Dcr 2 / Dicer 2 antibody (ab4732)
All lanes : Anti-Dcr 2 / Dicer 2 antibody (ab4732) at 10 µg/ml
Lane 1 : Drosophila Embryo Nuclear Extract (TRAX)
Lane 2 : Drosophila Embryo Nuclear Extract (TRAX) with Blocking peptide
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Predicted band size : 188 kDa
Observed band size : 36,190 kDa (why is the actual band size different from the predicted?)
References for Anti-Dcr 2 / Dicer 2 antibody (ab4732)
This product has been referenced in:
- Hartig JV & Förstemann K Loqs-PD and R2D2 define independent pathways for RISC generation in Drosophila. Nucleic Acids Res 39:3836-51 (2011). WB; Fruit fly (Drosophila melanogaster).Read more (PubMed: 21245036) »
- Mukherjee S & Hanley KA RNA interference modulates replication of dengue virus in Drosophila melanogaster cells. BMC Microbiol 10:127 (2010). WB; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20420715) »
See all 3 publications for this product
Publishing research using ab4732? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"