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i just wanted to know if it would be possible for me to test your polyclonal dicer antibody on pombe Dcr1. thanks!
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ANSWER: |
Thank you for your enquiry. At Abcam we always interested in improving the quality of information on our datasheets. To this effect I would be very interested in determining the cross species reactivity of ab4735. Please can you tell me the applications that you have in mind for this antisera (WB, ChIP etc.) I look forward to hearing from you. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lanes 1 - 2 : Anti-Dicer antibody (ab4735) at 1/500 dilution
Lanes 3 - 4 : Anti-Dicer antibody (ab4735) at 1/1000 dilution
Lane 1 : 5 ug of drosophila embryo lysate.
Lane 2 : 5 ug of drosophila embryo lysate.
Lane 3 : 5 ug of drosophila embryo lysate.
Lane 4 : 5 ug of drosophila embryo lysate.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 255 kDa
Observed band size : 255 kDa
Additional bands at : 170 kDa (possible cleavage fragment,cross reactivity).
Exposure time : 30 seconds
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