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Western blot - Dcr1 / Dicer 1 antibody (ab4735)
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Product Name
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Dicer antibody
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See all Dicer antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dicer
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Immunogen
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Synthetic peptide derived from residues 900 - 1000 Dicer (Dcr1).
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Reacts with
(species key)
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Dm
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Specificity
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There are multiple bands in the blot shown, though there is a band at the appropriate predicted size (255 kDa).
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/500. Detects a band of approximately 255 kDa (predicted molecular weight: 255 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Dicer
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 42693 (Fruit fly (Drosophila melanogaster))
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| SwissProt | | Q9VCU9 (Fruit fly (Drosophila melanogaster))
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dicer antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dicer antibody images, references, products related to ab4735 and other tools.
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Dicer antibody images:
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 Western blot - Dcr1 / Dicer 1 antibody (ab4735)
Lanes 1 - 2 : Dicer antibody (ab4735) at 1/500 dilution
Lanes 3 - 4 : Dicer antibody (ab4735) at 1/1000 dilution
Lane 1 : 5 ug of drosophila embryo lysate.
Lane 2 : 5 ug of drosophila embryo lysate.
Lane 3 : 5 ug of drosophila embryo lysate.
Lane 4 : 5 ug of drosophila embryo lysate.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab7090) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 255 kDa
Observed band size : 255 kDa
Additional bands at : 170 kDa (possible cleavage fragment,cross reactivity).
Exposure time : 30 seconds
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References for Dicer antibody (ab4735)
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This product has been used in: (two most recent references)
Xu XL et al. The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. J Neurosci 28:11883-9 (2008). PubMed: 19005053
Meyer WJ et al. Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. PLoS Genet 2:e134 (2006). PubMed: 16934003
See all 3 publication references for this product.
If you publish research using ab4735 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Dicer
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Dicer antibody - more information
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Customer reviews (feedback) regarding Dicer antibody |
Customer FAQs regarding Dicer antibody |
Protocols for Dicer antibody |
Price and availability of products related to Dicer antibody |
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Dicer antibody - related products:
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Dicer antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dicer antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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