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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Dicer antibody
See all Dicer products (11) ...
Description
Rabbit polyclonal to Dicer
Specificity
There are multiple bands in the blot shown, though there is a band at the appropriate predicted size (255 kDa).
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide derived from residues 900 - 1000 Dicer (Dcr1).
.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Dcr1 / Dicer 1 antibody (ab4735)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 255 kDa (predicted molecular weight: 255 kDa).
Target
Function
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Involvement in disease
Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:601200]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.
Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UPY3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dcr-1 antibody
- DCR1 antibody
- Dicer 1 ribonuclease type III antibody
see all
Database links
- Entrez Gene: 42693 Fruit fly (Drosophila melanogaster)
- SwissProt: Q9VCU9 Fruit fly (Drosophila melanogaster)
Anti-Dicer antibody images:
Western blot - Dcr1 / Dicer 1 antibody (ab4735)
Lanes 1 - 2 : Anti-Dicer antibody (ab4735) at 1/500 dilution
Lanes 3 - 4 : Anti-Dicer antibody (ab4735) at 1/1000 dilution
Lane 1 : 5 ug of drosophila embryo lysate.
Lane 2 : 5 ug of drosophila embryo lysate.
Lane 3 : 5 ug of drosophila embryo lysate.
Lane 4 : 5 ug of drosophila embryo lysate.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 255 kDa
Observed band size : 255 kDa
Additional bands at : 170 kDa (possible cleavage fragment,cross reactivity).
Exposure time : 30 seconds
References for Anti-Dicer antibody (ab4735)
This product has been referenced in:
- Kibanov MVet al. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Mol Biol Cell 22:3410-9 (2011). Fruit fly (Drosophila melanogaster).Read more (PubMed: 21775629) »
- Xu XLet al. The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. J Neurosci 28:11883-9 (2008).Read more (PubMed: 19005053) »
See all 4 publications for this product
Publishing research using ab4735? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"