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Anti-Deleted in azoospermia 4 antibody (ab69157)

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Overview

Product name

Anti-Deleted in azoospermia 4 antibody
See all Deleted in azoospermia 4 products (3) ...

Description

Mouse polyclonal to Deleted in azoospermia 4

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length human Deleted in azoospermia 4 protein (NP_065153)

Positive control

Deleted in azoospermia 4 transfected 293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Germline Stem Cells >> Spermatogonial Stem Cells
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Cell Biology >> Cell Cycle >> Cell differentiation

  • Western blot - Deleted in azoospermia 4 antibody (ab69157)Western blot - Deleted in azoospermia 4 antibody (ab69157) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: Isoform 2, 44 kDa; Isoform 1, 65kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Deleted in azoospermia 4 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that plays an essential role in spermatogenesis. It may act by binding to the 3'-UTR of mRNAs and regulating their translation.

Cellular localization

Cytoplasm. Nucleus.

Alternative names

  • DAZ4 antibody
  • Deleted in azoospermia 4 antibody
  • Deleted in azoospermia protein 4 antibody

Database links

Anti-Deleted in azoospermia 4 antibody images:

  Western blot - Deleted in azoospermia 4 antibody (ab69157)

Western blot - Deleted in azoospermia 4 antibody (ab69157)

All lanes : Anti-Deleted in azoospermia 4 antibody (ab69157) at 1/500 dilution

Lane 1 : Deleted in azoospermia 4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 44 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-Deleted in azoospermia 4 antibody (ab69157)

ab69157 has not yet been referenced specifically in any publications.

Publishing research using ab69157? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"