Recombinant Dengue Virus 2 NS1 glycoprotein (ab64456)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: ELISA, WB
Description
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Product name
Recombinant Dengue Virus 2 NS1 glycoprotein
See all Dengue Virus NS1 glycoprotein proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Dengue virus 2 -
Predicted molecular weight
48 kDa including tags -
Tags
His tag C-Terminus
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Description
Recombinant Dengue Virus 2 Dengue Virus NS1 glycoprotein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab64456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 48% Urea, 1.58% Tris HCl, 1.16% Sodium chloride
General Info
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Alternative names
- Dengue NS1
- Dengue Virus non structural protein 1
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Relevance
NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab64456 has been referenced in 7 publications.
- Coelho SVA et al. Contact System Activation in Plasma from Dengue Patients Might Harness Endothelial Virus Replication through the Signaling of Bradykinin Receptors. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 33445640
- Garrote BL et al. Field effect in molecule-gated switches and the role of target-to-receptor size ratio in biosensor sensitivity. Biosens Bioelectron 127:215-220 (2019). PubMed: 30616114
- Austin Suthanthiraraj PP & Sen AK Localized surface plasmon resonance (LSPR) biosensor based on thermally annealed silver nanostructures with on-chip blood-plasma separation for the detection of dengue non-structural protein NS1 antigen. Biosens Bioelectron 132:38-46 (2019). PubMed: 30851494
- Garrote BL et al. Perspectives on and Precautions for the Uses of Electric Spectroscopic Methods in Label-free Biosensing Applications. ACS Sens 4:2216-2227 (2019). PubMed: 31394901
- Wasik D et al. Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis. Sensors (Basel) 18:N/A (2018). PubMed: 30103543
- Cecchetto J et al. The capacitive sensing of NS1 Flavivirus biomarker. Biosens Bioelectron 87:949-956 (2017). PubMed: 27665517
- Chen J et al. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog 11:e1005053 (2015). PubMed: 26226614