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Overview

  • Product nameDengue Virus IgG Human ELISA Kit
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108728 Dengue Virus IgG Human ELISA Kit is intended for the qualitative determination of IgG class antibodies against Dengue Virus in Human serum or plasma (citrate).

    Dengue virus is a single-stranded RNA virus of about 50 nm in diameter belonging to the genus Flavivirus. Dengue and dengue hemorrhagic fever are caused by one of four closely related, but antigenically distinct, virus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). Infection with one of these serotypes does not provide crossprotective immunity, so persons living in a dengue-endemic area can have four dengue infections during their lifetimes. The viruses are transmitted by Aedes aegypti, a domestic, day-biting mosquito that prefers to feed on Humans. Infection with dengue viruses produces a spectrum of clinical illness ranging from a nonspecific viral syndrome to severe and fatal hemorrhagic disease. It is primarily a disease of the tropics; its global distribution is comparable to that of malaria, and an estimated 2.5 billion people live in areas at risk for epidemic transmission. - Globally, there are an estimated 50 to 100 million cases of dengue fever and several hundred thousand cases of dengue hemorrhagic fever.

  • Tested applicationsELISA more details

Properties

Applications

Our Abpromise guarantee covers the use of ab108728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA

Protocols

References for Dengue Virus IgG Human ELISA Kit (ab108728)

ab108728 has not yet been referenced specifically in any publications.

Publishing research using ab108728 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 7 of 7 results for Abreviews and Q&A

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I want to know about sample Serum, Plasma if my sample mix with EDTA. EDTA will effect with ELISA kit ?

i will use two kit for my sample.my sample is Serum, Plasma with EDTA

and abcam product i need is

Dengue Virus IgG Hum...

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The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guarante...

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Customer kindly contacted us regarding the specificity of ab108728 for Dengue Virus serotypes 1-4. He was specifically wondering if we had any data showing that the antibody in the kit recognizes all 4 serotypes.


I contacted the laboratory, and they confirmed that ab108728 does in fact cross react with Dengue Virus serotypes 1-4. It is very difficult to get samples with defined subtype of Dengue because the antibodies show a strong cross reactivity. Due to th...

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Does this ELISA kit able to detect antibody against all 4 serotypes? I believe plate is coated with DEN-2 antigen but I wonder if there is cross reactivity.

This product is not in stock and currently has a 1 -2 week lead time. The kit will recognize all four serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). While the Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Vi...

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Is it known whether this kit will cross react with West Nile virus too?

-Is it specific to Dengue type 2?

The Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4, thus the kit will detect IgG from patient samples past infected with any of the four se...

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Might you be able to provide information on the specific anti-Dengue antibody used as capture antibody in the ELISA, particularly the immunogenic sequence (ie sequence injected into mouse or rabbit to produce the Ab?)



In this assay, microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. No capture antibody is used. The only antibody in the kit is the anti-human-HRP conjugate used to detect any ...

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Is it known whether this kit will cross react with West Nile virus too?

We do not have cross reactivity data for West Nile Virus.

  Hola, quisiera saber que proteina usais para los kits comerciales, ya que veo que hay la posibilidad de la NS1 y la enveloped protein (para los 4 serotipos de virus). Muchas gracias anticipadas

Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ayu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"