Anti-Dengue virus IgG Human ELISA Kit (ab108728)

Overview

  • Product nameAnti-Dengue virus IgG Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 8 4.34%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Dengue virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Dengue virus in Human serum and plasma.

     

    A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgG sample captured in plate.

  • Tested applicationsIndirect ELISA more details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108728 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Anti-Dengue virus IgG Human ELISA Kit (ab108728)

    ab108728 has not yet been referenced specifically in any publications.

    Product Wall



    The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guara...

    Read More


    I contacted the laboratory, and they confirmed that ab108728 does in fact cross react with Dengue Virus serotypes 1-4. It is very difficult to get samples with defined subtype of Dengue because the antibodies show a strong cross reactivity. Due to...

    Read More

    This product is not in stock and currently has a 1 -2 week lead time. The kit will recognize all four serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). While the Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue...

    Read More

    The Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4, thus the kit will detect IgG from patient samples past infected with any of the four...

    Read More



    In this assay, microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. No capture antibody is used. The only antibody in the kit is the anti-human-HRP conjugate used to detect a...

    Read More

    We do not have cross reactivity data for West Nile Virus.

    Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"