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Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

Abreviews (1)Q&A (27)Specific References (2)

Overview

  • Product nameAnti-Dengue Virus NS1 glycoprotein antibody [DN3]See all Dengue Virus NS1 glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Dengue Virus 1, Dengue Virus 2, Dengue Virus 3, Dengue Virus 4
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

  • Positive controlIt is important to ensure that DEN4 infection is effective. DEN4 is notoriously difficult to grow to good titres and NS1 antigens will not be found if infection is not well established.

Properties

Applications

Our Abpromise guarantee covers the use of ab41616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 40, 80 kDa). Block membrane with PBS containing 5% non-fat skimmed milk (PBS-SM) for 30 minutes. Wash membrane and probe with serum diluted in PBS-SM with 10 mM sodium azide by incubating overnight on a rocker at room temperature.
ICC/IF ICC/IF: 1/5 - 1/20.

Target

  • RelevanceNS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • Database links
    • Alternative names
        Dengue NS1 antibodyDengue Virus non-structural protein 1 antibody

    Anti-Dengue Virus NS1 glycoprotein antibody [DN3] images

    • Western blot - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
      Western blot - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
      All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

      Lane 1 : Dengue Virus 1 infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 1 infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (boiled)
      Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate (unheated)
      Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate (boiled)
      Lane 7 : Dengue Virus 3 infected C6/36 cell lysate (unheated)
      Lane 8 : Dengue Virus 3 infected C6/36 cell lysate (boiled)
      Lane 9 : Dengue Virus 4 infected C6/36 cell lysate (unheated)
      Lane 10 : Dengue Virus 4 infected C6/36 cell lysate (boiled)


      Predicted band size : 40, 80 kDa
      Observed band size : 40,80 kDa
    • Immunocytochemistry/ Immunofluorescence - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
      Immunocytochemistry/ Immunofluorescence - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
      Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein NS1 (green). Cell nuclei are blue.
    • Immunocytochemistry/ Immunofluorescence - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
      Immunocytochemistry/ Immunofluorescence - Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)Image from Heaton NS et al, Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50. Epub 2010 Sep 20, Fig 2. DOI 10.1073/pnas.1010811107
      ab41616 staining NS1 glycoprotein in Dengue Virus 2 infected Huh-7.5 cells by Immunocytochemistry/ Immunofluorescence.

      Huh-7.5 cells were plated on glass coverslips, infected with Dengue Virus 2, fixed in methanol, and blocked, and antibody was added in 1× PBS + 0.1% saponin. Antibodies used include FASN polyclonal, left image (ab3844) or NS1 monoclonal antibody, middle image (ab41616). Secondary antibodies were species-appropriate Alexa-Fluor 488 or 594 secondary antibody. Stained coverslips were mounted with DAPI, right merged image.

    References for Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

    This product has been referenced in:
    • Clark KB  et al. Multiploid CD61+ cells are the pre-dominant cell lineage infected during acute dengue virus infection in bone marrow. PLoS One 7:e52902 (2012). ICC/IF ; Human . Read more (PubMed: 23300812) »
    • Heaton NS  et al. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107:17345-50 (2010). ICC/IF . Read more (PubMed: 20855599) »

    See all 2 Publications for this product

    Publishing research using ab41616 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab41616 Submit a Question for ab41616

    Displaying 1 - 10 of 28 results for Abreviews and Q&A

    10
    Votes: Highest First

    Our Customer has a doubt, What serotype of Dengue recognize antibody ab41616? The antibody say /DN3) and she think that is serotype 3?

    Thank you for contacting us.

    DN3 is just a clone name. The antibody is reactive to all serotypes.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Dear Abcam technical support,

    In the attachment is the abreviews that our customer from Siriraj Hospital previously discussed with us, that she will finish the reviews and submit to abcam in order to get the discount. Could u please kindly proc...

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    Thank you for your message which has been forwarded to me as my colleague Anja is currently out of the office.

    We appreciate the feedback from the customer, thank you for sending this information.

    In order to obtain the discount, the...

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    ELISA abreview for Anti-Dengue Virus NS1 glycoprotein antibody [DN3]

     Poor
    Abreviews
    Application ELISA
    Sample Escherichia coli Recombinant protein (Dengue NS1)
    Specification Dengue NS1
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Type Indirect
    Read More
    Username

    Verified customer

    Submitted Jul 26 2012

    Dear Sir/ Madam,
    We are trying to develop and ELISA for Dengue NS1 detection and having been working with your products in some of our previous and other present work, we have considered using your dengue products as well. However, during our prelimi...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable th...

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    Dear Technical support teams,

    Would you pls suggest me aboutantibody for dengue
    > > non-structural protein 1 (NS1) , that be able to use in ELISA method.

    The customer found only the anti-dengue NS1 for ICF or WB applicatio...

    Read More

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in EL...

    Read More

    Thanks for the mail information, Please find that we need below kits.


    Dengue NS1 Antibodies.

    Isotype Controls Kit.


    Please inform its literature prices and expiry. Are you accept payment by credit card?

    Read More

    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glycopr...

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    Buenos días ,
    te hago llegar los comentarios de mis compañeros.
    We will very much appreciate if you can provide us details on the epitope targeted byab41616 and also if it has been compromised in the his tag region ofab64456 so we ...

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    Gracias por tu respuesta.
    He consultado esta información con mis compañeros del laboratorio.
    La glicoproteína (ab64456) contiene toda la secuencia excepto el dominio transmembranal. Además contiene 2x6 His tags, uno localizado en el extremo N te...

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    Dear All,



    Please let us know which Isotype control can be offered with the Dengue NS1 (ab41616) antibody.



    Early response will be appreciated.


    Sincere regards,

    Thank you for your inquiry.

    ab41616 is a mouse monoclonal IgG1 with a kappa light chain, un-conjugated.

    Therefore a isotype control is needed that is also un-conjugated and of the IgG1 Isotype (preferably with a kappa light chain). Her...

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    Dear Sir/Madam,
    We are trying to develop an ELISA for Dengue NS1 detection and since we have been been working for a long time with your products, we have decided to use your dengue products. We are in the process of validating our immunocapture syst...

    Read More

    Thank you for contacting us.
    I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, but ...

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    I am looking for a system of a Antigen- Antibody system to couple it with carboxilated magnetic beads. I was thinking that the Anti-Dengue Virus NS1 glycoprotein antibody [DN3] and theVirus NS1 glycoproteinare suitable candidates for our purposes. Neverth...

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    Thank you very much for your interest in ab41616, anti-Dengue Virus NS1 glycoprotein antibody [DN3].

    We have a general protocol for the crosslinking antibodies toProtein A/Gbeads : http://www.abcam.com/index.html?pageconfig=resource&rid=1141...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"