Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

Overview

  • Product nameAnti-Dengue Virus NS1 glycoprotein antibody [DN3]See all Dengue Virus NS1 glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Dengue Virus 1, Dengue Virus 2, Dengue Virus 3, Dengue Virus 4
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

  • Positive control
    • It is important to ensure that DEN4 infection is effective. DEN4 is notoriously difficult to grow to good titres and NS1 antigens will not be found if infection is not well established.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 10mM Sodium Azide
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberDN3
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab41616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 40, 80 kDa). Block membrane with PBS containing 5% non-fat skimmed milk (PBS-SM) for 30 minutes. Wash membrane and probe with serum diluted in PBS-SM with 10 mM sodium azide by incubating overnight on a rocker at room temperature.
ICC/IF 1/5 - 1/20.

Target

  • RelevanceNS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • Database links
    • Alternative names
      • Dengue NS1 antibody
      • Dengue Virus non-structural protein 1 antibody

    Anti-Dengue Virus NS1 glycoprotein antibody [DN3] images

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

      Lane 1 : Dengue Virus 1 infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 1 infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (boiled)
      Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate (unheated)
      Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate (boiled)
      Lane 7 : Dengue Virus 3 infected C6/36 cell lysate (unheated)
      Lane 8 : Dengue Virus 3 infected C6/36 cell lysate (boiled)
      Lane 9 : Dengue Virus 4 infected C6/36 cell lysate (unheated)
      Lane 10 : Dengue Virus 4 infected C6/36 cell lysate (boiled)


      Predicted band size : 40, 80 kDa
      Observed band size : 40,80 kDa
    • Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein NS1 (green). Cell nuclei are blue.
    • ab41616 staining NS1 glycoprotein in Dengue Virus 2 infected Huh-7.5 cells by Immunocytochemistry/ Immunofluorescence.
      Huh-7.5 cells were plated on glass coverslips, infected with Dengue Virus 2, fixed in methanol, and blocked, and antibody was added in 1× PBS + 0.1% saponin. Antibodies used include FASN polyclonal, left image (ab3844) or NS1 monoclonal antibody, middle image (ab41616). Secondary antibodies were species-appropriate Alexa-Fluor 488 or 594 secondary antibody. Stained coverslips were mounted with DAPI, right merged image.

    References for Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

    This product has been referenced in:
    • Prompetchara E  et al. Induction of neutralizing antibody response against four dengue viruses in mice by intramuscular electroporation of tetravalent DNA vaccines. PLoS One 9:e92643 (2014). ICC/IF . Read more (PubMed: 24887426) »
    • Thepparit C  et al. Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death. BMC Res Notes 6:372 (2013). WB . Read more (PubMed: 24034452) »

    See all 4 Publications for this product

    Product Wall

    Application Flow Cytometry
    Fixation BD CytoFix/CytoPerm
    Permeabilization Yes - BD CytoFix/CytoPerm
    Sample Human Cell (HeLa at 62hpi with DV2)
    Specification HeLa at 62hpi with DV2
    Gating Strategy single cells by FSC and SSC
    Preparation Cell harvesting/tissue preparation method: spatula scraping
    Sample buffer: cold PBS
    Username

    Lee Gehrke

    Verified customer

    Submitted Oct 07 2013

    Thank you for contacting us.

    DN3 is just a clone name. The antibody is reactive to all serotypes.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your message which has been forwarded to me as my colleague Anja is currently out of the office.

    We appreciate the feedback from the customer, thank you for sending this information.

    In order to obtain the discount, ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application ELISA
    Sample Escherichia coli Recombinant protein (Dengue NS1)
    Specification Dengue NS1
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Type Indirect
    Username

    Abcam user community

    Verified customer

    Submitted Jul 26 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in...

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    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glyc...

    Read More

    Gracias por tu respuesta.
    He consultado esta información con mis compañeros del laboratorio.
    La glicoproteína (ab64456) contiene toda la secuencia excepto el dominio transmembranal. Además contiene 2x6 His tags, uno localizado en el extremo N...

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    Thank you for your inquiry.

    ab41616 is a mouse monoclonal IgG1 with a kappa light chain, un-conjugated.

    Therefore a isotype control is needed that is also un-conjugated and of the IgG1 Isotype (preferably with a kappa light chain). ...

    Read More

    Thank you for contacting us.
    I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, b...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"