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Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
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Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
(enlarge)


Product Name 

Dengue Virus prM glycoprotein antibody [DM1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein

Immunogen 

Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

Reacts with 
(species key)

DV2

Specificity 

This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.

Tested applications 
(see key)

ELISA, ICC/IF, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/5 - 1/20.
WB: 1/50 - 1/100. Use under non reducing conditions. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Dengue

Relevance 

There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.

 

Alternative names Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DM1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 10mM Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dengue Virus prM glycoprotein antibody [DM1] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Dengue Virus prM glycoprotein antibody [DM1] images, references, products related to ab41473 and other tools.

 

Dengue Virus prM glycoprotein antibody [DM1] images:

Product image  Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

All lanes : Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Lane 1 : Dengue Virus 1 infected C6/36 cell lysate
Lane 2 : Dengue Virus 1 infected C6/36 cell lysate
Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 7 : Dengue Virus 3 infected C6/36 cell lysate
Lane 8 : Dengue Virus 3 infected C6/36 cell lysate
Lane 9 : Dengue Virus 4 infected C6/36 cell lysate
Lane 10 : Dengue Virus 4 infected C6/36 cell lysate


Predicted band size : 21 kDa
Observed band size : 21 kDa

 

Product image  Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

 
 

Search PubMed (MEDLINE) for references to Dengue Virus prM glycoprotein

 

Dengue Virus prM glycoprotein antibody [DM1] - more information

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