Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Overview

  • Product name
    Anti-Dengue Virus prM glycoprotein antibody [DM1]
  • Description
    Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
  • Specificity
    This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Dengue virus 2
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    DM1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20.
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
  • Alternative names
    • pre membrane glycoprotein antibody
    • pre-membrane glycoprotein antibody

Anti-Dengue Virus prM glycoprotein antibody [DM1] images

  • All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

    Lane 1 : Dengue Virus 1 infected C6/36 cell lysate
    Lane 2 : Dengue Virus 1 infected C6/36 cell lysate
    Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lane 7 : Dengue Virus 3 infected C6/36 cell lysate
    Lane 8 : Dengue Virus 3 infected C6/36 cell lysate
    Lane 9 : Dengue Virus 4 infected C6/36 cell lysate
    Lane 10 : Dengue Virus 4 infected C6/36 cell lysate


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

References for Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

This product has been referenced in:
  • Huang X  et al. Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy. Sci Rep 6:22303 (2016). Read more (PubMed: 26923481) »
  • Palma-Ocampo HK  et al. Interferon lambda inhibits dengue virus replication in epithelial cells. Virol J 12:150 (2015). ICC/IF . Read more (PubMed: 26411318) »

See all 3 Publications for this product

Product Wall

Q1. What cell type was infected with the virus in order to produce the immunogen?
A1: The mosquito cell line, C6/36, is used for growing virus.

Q2. How was the immunogen purified?
A2: Infected supernatant and lysate were used as immun...

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J’ai contacté le laboratoire qui m’a envoyé les résultants ELISA, voir pièce jointe.

Pour le western blot, les lysats de cellules C6/36 infectés ont été dilués dans...

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Thank you for your enquiry. This antibody recognizes all 4 serotypes in ELISA, however in Western blot application it detects only Dengue 2. I hope this information will be useful for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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