Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Overview
- Product nameAnti-Dengue Virus prM glycoprotein antibody [DM1]
- DescriptionMouse monoclonal [DM1] to Dengue Virus prM glycoprotein
- SpecificityThis antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
- Tested applicationsICC/IF, WB, ELISA more details
- Species reactivityReacts with: Dengue Virus 2
- Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.065% Sodium azide
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberDM1
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab41473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/5 - 1/20. |
| WB | WB: 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- RelevanceThere are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
-
Alternative names
- pre membrane glycoprotein antibodypre-membrane glycoprotein antibody
Anti-Dengue Virus prM glycoprotein antibody [DM1] images
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All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Lane 1 : Dengue Virus 1 infected C6/36 cell lysate
Lane 2 : Dengue Virus 1 infected C6/36 cell lysate
Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 7 : Dengue Virus 3 infected C6/36 cell lysate
Lane 8 : Dengue Virus 3 infected C6/36 cell lysate
Lane 9 : Dengue Virus 4 infected C6/36 cell lysate
Lane 10 : Dengue Virus 4 infected C6/36 cell lysate
Predicted band size : 21 kDa
Observed band size : 21 kDa -
Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).
References for Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
This product has been referenced in:
- Liang Z et al. Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-ß in cultured hepatoma cells. PLoS One 6:e23346 (2011). ICC/IF ; Dengue Virus 2 . Read more (PubMed: 21829730) »
![Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473) Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)](http://a.abcam.com/ps/datasheet/Images/41/ab41473/ab41473_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473) Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)](http://a.abcam.com/ps/datasheet/Images/41/ab41473/ab41473_2.jpg)