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Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameAnti-Dengue Virus prM glycoprotein antibody [DM1]
  • Description
    Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
  • SpecificityThis antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
  • Tested applicationsICC/IF, WB, ELISA more details
  • Species reactivity
    Reacts with: Dengue Virus 2
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

Properties

Applications

Our Abpromise guarantee covers the use of ab41473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/5 - 1/20.
WB WB: 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA ELISA: Use at an assay dependent dilution.

Target

  • RelevanceThere are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
  • Alternative names
      pre membrane glycoprotein antibodypre-membrane glycoprotein antibody

Anti-Dengue Virus prM glycoprotein antibody [DM1] images

  • Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

    Lane 1 : Dengue Virus 1 infected C6/36 cell lysate
    Lane 2 : Dengue Virus 1 infected C6/36 cell lysate
    Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lane 7 : Dengue Virus 3 infected C6/36 cell lysate
    Lane 8 : Dengue Virus 3 infected C6/36 cell lysate
    Lane 9 : Dengue Virus 4 infected C6/36 cell lysate
    Lane 10 : Dengue Virus 4 infected C6/36 cell lysate


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

References for Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

This product has been referenced in:
  • Liang Z  et al. Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-ß in cultured hepatoma cells. PLoS One 6:e23346 (2011). ICC/IF ; Dengue Virus 2 . Read more (PubMed: 21829730) »

See 1 Publication for this product

Publishing research using ab41473 ? Please let us know so that we can cite the reference in this datasheet

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question sur ab41473.



J’ai contacté le laboratoire qui m’a envoyé les résultants ELISA, voir pièce jointe.

Pour le western blot, les lysats de cellules C6/36 infectés ont été dilués dans un...

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Dear Sir. or Madam I try to order the anti-prM antibody (Code:ab41473). I try to make sure whether the antibody cross react with DENV1, 3, 4? The datasheet reads it recognize DENV 1,2,3,4, but the WB pricture shows it only react with DENV2. Thanks...

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Thank you for your enquiry. This antibody recognizes all 4 serotypes in ELISA, however in Western blot application it detects only Dengue 2. I hope this information will be useful for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"