![Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473) image](#ab41473_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473) image](#ab41473_2.jpg)
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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Dengue
Overview
Product name
Anti-Dengue Virus prM glycoprotein antibody [DM1]
Description
Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
Specificity
This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Dengue Virus 2
Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 10mM Sodium Azide
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
DM1
Isotype
IgG1
Light chain type
kappa
Research areas
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Dengue
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/5 - 1/20.
WB: 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA: Use at an assay dependent dilution.
Target
Relevance
There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
Alternative names
- pre membrane glycoprotein antibody
- pre-membrane glycoprotein antibody
Anti-Dengue Virus prM glycoprotein antibody [DM1] images:
Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
![Western blot - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)](/ps/datasheet/Images/41/ab41473/ab41473_1.jpg)
All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Lane 1 : Dengue Virus 1 infected C6/36 cell lysate
Lane 2 : Dengue Virus 1 infected C6/36 cell lysate
Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lane 7 : Dengue Virus 3 infected C6/36 cell lysate
Lane 8 : Dengue Virus 3 infected C6/36 cell lysate
Lane 9 : Dengue Virus 4 infected C6/36 cell lysate
Lane 10 : Dengue Virus 4 infected C6/36 cell lysate
Predicted band size : 21 kDa
Observed band size : 21 kDa
Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
![Immunocytochemistry/ Immunofluorescence - Dengue Virus prM glycoprotein antibody [DM1] (ab41473)](/ps/datasheet/Images/41/ab41473/ab41473_2.jpg)
Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).
References for Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
This product has been referenced in:
- Liang Zet al. Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-ß in cultured hepatoma cells. PLoS One 6:e23346 (2011). ICC/IF; Dengue Virus 2.Read more (PubMed: 21829730) »
See 1 publication for this product
Publishing research using ab41473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"