Desmin antibody [DE-U-10] (ab6322)

Product Name 

Desmin antibody [DE-U-10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DE-U-10] to Desmin

Immunogen 

Full length native protein (purified) (Pig).

Reacts with 
(species key)

Hu, Ms, Rat, Cat, Chk, Cow, Goat, Hm, Lzd, Rb, Shp, Snk

Specificity 

This antibody is specific for desmin as determined by indirect immunofluorescent staining methods. It specifically stains the wide desmin band of 50,000 to 55,000 molecular weight by immunoblotting methods.

Tested applications 
(see key)

ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-F: Use at an assay dependant dilution.
ICC: Use at an assay dependant dilution.
IF: 1/20.
IHC-P: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Monoclonal anti-desmin may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Desmin

Relevance 

Desmins belongs to the intermediate filament family, and are class III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy (CSM) also known as desmin related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin reactive deposits in cardiac and skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). Defects in Desmin are also the cause of dilated cardiomyopathy type 1I (CMD1I). CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DE-U-10

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Ascitic fluid with 15mM sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 8.30 - 8.80 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Desmin antibody [DE-U-10] (ab6322)

  This product has been used in: (two most recent references)

Davis DR et al. Validation of the cardiosphere method to culture cardiac progenitor cells from myocardial tissue. PLoS One 4:e7195 (2009). PubMed: 19779618

Khaleduzzaman M et al. Infection of cardiomyocytes and induction of left ventricle dysfunction by neurovirulent polytropic murine retrovirus. J Virol 81:12307-15 (2007). IHC-P; Mouse. PubMed: 17855522

See all 3 publication references for this product.


If you publish research using ab6322 please let us know so that we can cite the reference on this datasheet.

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