Immunohistochemistry (Paraffin-embedded sections) - Desmin antibody (ab15200)

Ab15200 positively staining desmin in formaldehyde fixed mouse heart tissue (1/200). Ab15200 was used in conjunction with goat anti rabbit (biotin).

The reviewer reported no difference in the quality of staining obtained when enzymatic epitope unmasking was preformed.

This image is an edited version of an image submitted courtesy of an Abreview by Khaled Chatila on 11 October 2005. For further information relating to the protocol please refer to the Abreview.

Immunohistochemistry (Frozen sections) - Desmin antibody (ab15200)

ab15200 at a 1/200 dilution staining mouse aortic root tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 10% serum then incubated with antibody for 30 minutes. Bound antibody was detected using a biotinylated goat anti-rabbit antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Desmin antibody (ab15200)

ab15200 staining rat differentiated skeletal muscle cells by ICC/IF.

Cultured differentiated skeletal muscle cells were harvested from Lewis rats, 2% paraformaldehyde treated for 15 min to fix the cells, and then permealized with Triton-X100 (0.1%) for 10 min. The cells were then incubated with ab15200 at 1/200 overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows desmin expressing skeletal myocytes (green-cytoplasmic localization). Note that desmin forms short thickened filamentous structures in the cell cytoplasm, along with prominent spot-like cytoplasmic aggregates that are composed of densely packed filaments.

This image is courtesy of an Abreview submitted by Dr Mal Niladri

Western blot - Desmin antibody (ab15200)

Anti-Desmin antibody (ab15200) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 52 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 72 kDa (possible non-specific binding).

Immunocytochemistry/ Immunofluorescence - Desmin antibody (ab15200)

ab15200 staining Desmin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in PSS for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20⁰C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An MFP 555-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/400 dilution.

This image is courtesy of an anonymous Abreview