Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Desmin
Overview
Product name
Anti-Desmin antibody
See all Desmin products (19) ...
Description
Rabbit polyclonal to Desmin
Tested applications
IHC-Fr, Flow Cyt, ICC/IF, WB, IHC-P, IP, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).
Positive control
Muscle or Sarcoma. (Negative control: skin squamous cells).
General notes
The negative control used to test this Ab was: skin(squamous epithelial cells)was used as negative control tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac arrhythmias
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Desmin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (Used at a dilution of 1/200 for 30 min on mouse aortic root (see Abreview for further details).)
Flow Cyt: Use at an assay dependent dilution. (PubMed: 20091346)
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
IHC-P: 1/200
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Involvement in disease
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
Sequence similarities
Belongs to the intermediate filament family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP17661
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
Database links
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all
Anti-Desmin antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Desmin antibody (ab15200)
Ab15200 positively staining desmin in formaldehyde fixed mouse heart tissue (1/200). Ab15200 was used in conjunction with goat anti rabbit (biotin).
The reviewer reported no difference in the quality of staining obtained when enzymatic epitope unmasking was preformed.
This image is an edited version of an image submitted courtesy of an Abreview by Khaled Chatila on 11 October 2005. For further information relating to the protocol please refer to the Abreview.
Immunohistochemistry (Frozen sections) - Desmin antibody (ab15200)
ab15200 at a 1/200 dilution staining mouse aortic root tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 10% serum then incubated with antibody for 30 minutes. Bound antibody was detected using a biotinylated goat anti-rabbit antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Desmin antibody (ab15200)
ab15200 staining rat differentiated skeletal muscle cells by ICC/IF.
Cultured differentiated skeletal muscle cells were harvested from Lewis rats, 2% paraformaldehyde treated for 15 min to fix the cells, and then permealized with Triton-X100 (0.1%) for 10 min. The cells were then incubated with ab15200 at 1/200 overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows desmin expressing skeletal myocytes (green-cytoplasmic localization). Note that desmin forms short thickened filamentous structures in the cell cytoplasm, along with prominent spot-like cytoplasmic aggregates that are composed of densely packed filaments.
This image is courtesy of an Abreview submitted by Dr Mal Niladri
Western blot - Desmin antibody (ab15200)
Anti-Desmin antibody (ab15200) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 52 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 72 kDa (possible non-specific binding).
Immunocytochemistry/ Immunofluorescence - Desmin antibody (ab15200)
ab15200 staining Desmin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in PSS for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 200C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An MFP 555-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/400 dilution.
This image is courtesy of an anonymous Abreview
References for Anti-Desmin antibody (ab15200)
This product has been referenced in:
- Ayala Ret al. Engineering the cell-material interface for controlling stem cell adhesion, migration, and differentiation. Biomaterials 32:3700-11 (2011). ICC/IF; Human.Read more (PubMed: 21396708) »
- Chung Iet al. De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation. J Cell Sci 124:3603-18 (2011).Read more (PubMed: 22045732) »
See all 10 publications for this product
Publishing research using ab15200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"