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Overview

  • Product nameDestrin protein (Tagged-His Tag)See all Destrin proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-165 of Human Destrin with an N terminal His tag. Predicted MWt: 20 kDa (UniProt ID: P60981).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 102 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesMASGVQVADEVCRIFYDMKVRKCSTPEEIKKRKKAVIFCL SADKKCIIV EEGKEILVGDVGVTITDPFKHFVGMLPEK DCRYALYDASFETKESRKEE LMFFLWAPELAPLKSKMI YASSKDAIKKKFQGIKHECQANGPEDLNRAC IAEKLGG SLIVAFEGCPV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92009 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        2610043P17RikACTDPActin depolymerizing factor
        Actin-depolymerizing factorADFAU042046bA462D18.2corn1DEST_HUMANDestrinDestrin (actin depolymerizing factor)DSNDSTNSid 23sid23p
      see all
  • FunctionActin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications    ISGylated.

    • Target information above from: UniProt accession P60981 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Destrin protein (Tagged-His Tag) images

    • SDS-PAGE - Destrin protein (Tagged-His Tag) (ab92009)
      SDS-PAGE - Destrin protein (Tagged-His Tag) (ab92009)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Destrin protein (Tagged-His Tag) (ab92009)

    ab92009 has not yet been referenced specifically in any publications.

    Publishing research using ab92009 ? Please let us know so that we can cite the reference in this datasheet

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