Dicer antibody [13D6] - ChIP Grade (ab14601)

Product Name 

Dicer antibody [13D6] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [13D6] to Dicer - ChIP Grade

Immunogen 

Synthetic peptide (Human).

Reacts with 

Hu, Ms, Rat

Does not react with 
(species key)

Ce

Specificity 

It is capable of recognising three proteines predicted by Aceview: the full length protein ( 1922 aa (218.7 kDa) issued from variants "a","b" et "c", a protein of 997 aa (113.2 kDa) issued from the transcriptional variant "d" and a protein of 820 aa (92.7 kDa) issued from variant transcriptionel "e".

Tested applications 
(see key)

ChIP, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC: 1/20 - 1/100.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18327211).
WB: 1/100 - 1/2000. Predicted molecular weight: 217 kDa.

Predicted molecular weight: 217 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: 10ng of recombinant dicer (using ECL or ECL Plus) in under one minute of exposure to film.
ICC: Human Fibroblasts, Mouse embryonic fibroblasts

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> RNAi >> Dicer
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi

Relevance 

RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

13D6

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

Batch dependent within range: 1.00 - 1.37 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

To our knowledge the localization of Dicer remains to be fully determined and it appears that its localization can be nuclear and/or cytoplasmic. Please see reference Meltzer, 2005 for a reference to a cytoplasmic localisation (figure 1 of paper), as supported with the staining seen in the image on this datasheet.

See below for Dicer antibody [13D6] - ChIP Grade images, references, products related to ab14601 and other tools.

Dicer antibody [13D6] - ChIP Grade images:

References for Dicer antibody [13D6] - ChIP Grade (ab14601)

  This product has been used in: (two most recent references)

Chen TS et al. Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs. Nucleic Acids Res 38:215-24 (2010). WB; Human. PubMed: 19850715

Grelier G et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer 101:673-83 (2009). IHC-P; Human. PubMed: 19672267

See all 22 publication references for this product.


If you publish research using ab14601 please let us know so that we can cite the reference on this datasheet.

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