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Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

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Overview

Product name

Anti-Dicer antibody [13D6] - ChIP Grade
See all Dicer products (11) ...

Description

Mouse monoclonal [13D6] to Dicer - ChIP Grade

Tested applications

ICC, ICC/IF, IHC-P, WB, Gel supershift assays, ChIPmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Caenorhabditis elegans

Immunogen

Synthetic peptide (Human).

Positive control

WB: 10ng of recombinant dicer (using ECL or ECL Plus) in under one minute of exposure to film. ICC: Human Fibroblasts, Mouse embryonic fibroblasts

General notes

To our knowledge the localization of Dicer remains to be fully determined and it appears that its localization can be nuclear and/or cytoplasmic. Please see reference Meltzer, 2005 for a reference to a cytoplasmic localisation (figure 1 of paper), as supported with the staining seen in the image on this datasheet.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Clonality

Monoclonal

Clone number

13D6

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> RNAi >> Dicer
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi

  • Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601) image (enlarge)

  • ChIP - Dicer antibody [13D6] (ab14601)ChIP - Dicer antibody [13D6] (ab14601) image (enlarge)

  • Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab14601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Involvement in disease

Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:601200]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.
Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionQ9UPY3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DCR1 antibody
  • Dcr1 homolog (Drosophila) antibody
  • Dicer 1 antibody
  • Dicer 1 ribonuclease type III antibody
  • Dicer antibody
  • DICER_HUMAN antibody
  • Dicer1 antibody
  • Endoribonuclease Dicer antibody
  • Helicase moi antibody
  • Helicase with RNAse motif antibody
  • HERNA antibody
  • KIAA0928 antibody
see all

Database links

Anti-Dicer antibody [13D6] - ChIP Grade images:

  Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)

Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)

Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with anti-Dicer antibody  ab14601 (1:100 dilution) o/n. The antibody showed mainly cytoplasmic staining.

Petra Hajkova, University of Cambridge

  ChIP - Dicer antibody [13D6] (ab14601)

ChIP - Dicer antibody [13D6] (ab14601)

ChIP analysis of Dicer protein binding at chicken ß-globin regulatory elements. Chromatin from human erythroleukemia (K562) cells containing chicken chromosomes with normal (FRK2) or mutant (HS1-K) ß-globin loci and chicken DT40 cells was immunprecipitated with antibodies to Dicer (ab14601). PCR analysis of immunoprecipitated chromatin was carried out using primers to the chicken ß-globin regulatory elements. Mouse IgG served as control. 10% of input DNA was utilized.

Elliot Epner MD PhD, Oregon Health and Science University, Portland

  Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)

Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)

All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/ml

Lane 1 : human B cell lymphoma lysate
Lane 2 : human B cell lymphoma lysate infected with control shRNA retrovirus
Lane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virus

Lysates/proteins at 2.5 µg/ml per lane.


Observed band size : 225 kDa (why is the actual band size different from the predicted?)

Jing Huang and Elliot Epner Oregon Health and Science University

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dicer antibody [13D6] - ChIP Grade (ab14601)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dicer antibody [13D6] - ChIP Grade (ab14601)

ab14601 staining Dicer in mouse ear tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in sodium citrate and then blocking with 5% serum was performed for 2 hours at 25°C. The primary antibody was diluted 1/100 and incubated with sample for 20 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/200 as secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

This product has been referenced in:

  • Inchley CSet al. Reduced Dicer expression in the cord blood of infants admitted with severe respiratory syncytial virus disease. BMC Infect Dis 11:59 (2011).Read more (PubMed: 21385408) »
  • Moore HCet al. An evolutionarily conserved, alternatively spliced, intron in the p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA. RNA 17:555-62 (2011).Read more (PubMed: 21346006) »

See all 31 publications for this product

Publishing research using ab14601? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"