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Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)
![Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)](/ps/datasheet/images/ab14601_4.jpg)
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ChIP - Dicer antibody [13D6] (ab14601)
![ChIP - Dicer antibody [13D6] (ab14601)](/ps/datasheet/images/ab14601_5.jpg)
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Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)
![Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)](/ps/datasheet/images/ab14601_7.jpg)
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Product Name
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Dicer antibody [13D6] - ChIP Grade
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See all Dicer antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [13D6] to Dicer - ChIP Grade
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Immunogen
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Synthetic peptide (Human).
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Reacts with
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Hu, Ms, Rat
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Does not react with
(species key)
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Ce
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Specificity
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It is capable of recognising three proteines predicted by Aceview: the full length protein ( 1922 aa (218.7 kDa) issued from variants "a","b" et "c", a protein of 997 aa (113.2 kDa) issued from the transcriptional variant "d" and a protein of 820 aa (92.7 kDa) issued from variant transcriptionel "e".
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Tested applications
(see key)
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ChIP, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ChIP: Use at an assay dependent dilution. ICC: 1/20 - 1/100. ICC/IF: Use at an assay dependent concentration. IHC-P: Use at an assay dependent dilution (PMID 18327211). WB: 1/100 - 1/2000. Predicted molecular weight: 217 kDa.
Predicted molecular weight: 217 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: 10ng of recombinant dicer (using ECL or ECL Plus) in under one minute of exposure to film. ICC: Human Fibroblasts, Mouse embryonic fibroblasts
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies Chromatin and Nuclear Signaling >> RNAi >> Dicer Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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13D6
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None Constituents: PBS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 1.37 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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To our knowledge the localization of Dicer remains to be fully determined and it appears that its localization can be nuclear and/or cytoplasmic. Please see reference Meltzer, 2005 for a reference to a cytoplasmic localisation (figure 1 of paper), as supported with the staining seen in the image on this datasheet.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dicer antibody [13D6] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dicer antibody [13D6] - ChIP Grade images, references, products related to ab14601 and other tools.
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Dicer antibody [13D6] - ChIP Grade images:
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Immunofluorescence - Dicer antibody [13D6] - ChIP Grade (ab14601)
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with anti-Dicer antibody ab14601 (1:100 dilution) o/n. The antibody showed mainly cytoplasmic staining. Petra Hajkova, University of Cambridge
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ChIP - Dicer antibody [13D6] (ab14601)
ChIP analysis of Dicer protein binding at chicken ß-globin regulatory elements. Chromatin from human erythroleukemia (K562) cells containing chicken chromosomes with normal (FRK2) or mutant (HS1-K) ß-globin loci and chicken DT40 cells was immunprecipitated with antibodies to Dicer (ab14601). PCR analysis of immunoprecipitated chromatin was carried out using primers to the chicken ß-globin regulatory elements. Mouse IgG served as control. 10% of input DNA was utilized. Elliot Epner MD PhD, Oregon Health and Science University, Portland
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Western blot - Dicer antibody [13D6] - ChIP Grade (ab14601)
All lanes : Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/ml
Lane 1 : human B cell lymphoma lysate Lane 2 : human B cell lymphoma lysate infected with control shRNA retrovirus Lane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virus
Lysates/proteins at 2.5 µg/ml per lane.
Predicted band size : 217 kDa Observed band size : 225 kDa (why is the actual band size different from the predicted?)
Jing Huang and Elliot Epner Oregon Health and Science University
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References for Dicer antibody [13D6] - ChIP Grade (ab14601)
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This product has been used in: (two most recent references)
Chen TS et al. Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs. Nucleic Acids Res 38:215-24 (2010). WB; Human. PubMed: 19850715
Grelier G et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer 101:673-83 (2009). IHC-P; Human. PubMed: 19672267
See all 22 publication references for this product.
If you publish research using ab14601 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Dicer
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Dicer antibody [13D6] - ChIP Grade - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"