Anti-Dicer antibody (ab13502)

Thanks very much, the antibody arrived today! Have a nice weekend,

ANSWER:

I hope this new antibody works for you not please do not hesitate to contact if you experience any issues with it.

    You can use the address you have (the one where the original antibody was shipped to), it should be correct. Thanks for your help, I appreciate it

ANSWER:

I have processed your request to receive ab13502 as a replacement. Your new order number is ******.

Please let me know if there is anything else I can help you with.

Yes, we would like to test another antibody: ab13502 is our choice. Please ship to:  

ANSWER:

Thank you for your reply.

I will be happy to send you ab13502 as a replacement. I would just like to confirm the address though, as the one you gave is slightly different to the one where the original antibody was shipped to. The address I have is: I know the addresses are only slightly different but I just want to make sure before I send the replacement.

Dear John,   please let me know, if you have any further information about your dicer antibody. I am a bit desperate to get more information / another antibody as our work is currently depending on these WB.  

ANSWER:

I still have not heard anything back from the lab about the specificity of the 75kDa band. From what I have seen with the other Dicer antibodies in our catalogue and from other readings it does seem that the Dicer antibodies in general do recognize a band at 75kDa.\ I would be happy to send you another Dicer antibody from our catalogue if that would help you interpret your results. If you would like to test another antibody could you please let me know which one?

I did another WB using your antibody. Again, I was looking for dicer in mouse kidneys. I used BSA 5% for blocking this time. The amount of protein per lane was the same as in the other WB before. Unfortunately, I don't get any band at 220kDa, only the one at 75kDa. I have attached the original .tiff-files with the integrated protein ladder for dicer.   If you have any other suggestions, I am very willing to try them. Otherwise, I would ask you to suggest another antibody that we can use.  

ANSWER:

I am currently in contact with the lab to see if they have any information about what the 75kDa band could be. I will contact you as soon as I get a reply from them. If we cannot determine what the 75kDa band is, then I would be happy to suggest another antibody that would be suitable.