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- Dicer antibody (ab13502)
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Western blot - Dicer antibody (ab13502)
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Product Name
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Dicer antibody
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See all Dicer antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Dicer
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Immunogen
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Synthetic peptide (Mouse), corresponding to amino acids FTKHQVLIMTCYVALTVLK (132-150) and QNKQILVPELCAIHPI (1017 -1033).
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Reacts with
(species key)
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Hu, Ms
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 217 kDa in whole ovary tissue lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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mouse ovary tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Dicer
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dicer antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Dicer antibody images, references, products related to ab13502 and other tools.
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Dicer antibody images:
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 - Dicer antibody (ab13502)
ab13502 at a 1/1000 dilution staining approximately 217kDa Dicer in 15µg of mouse ovary tissue lysate by western blot (ECL).
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Western blot - Dicer antibody (ab13502)
Dicer antibody (ab13502) at 1/1000 dilution + Human HeLa cell, whole cell lysate.
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 218 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa (possible non-specific binding).
Exposure time : 3 minutes This image is courtesy of an Abreview submitted by Dr Andrey Poleshko
See Abreview
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References for Dicer antibody (ab13502)
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This product has been used in: (two most recent references)
Grelier G et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer 101:673-83 (2009). WB; Human. PubMed: 19672267
Chen JF et al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A 105:2111-6 (2008). WB; Mouse. PubMed: 18256189
See all 6 publication references for this product.
If you publish research using ab13502 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Dicer
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Dicer antibody - more information
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Customer reviews (feedback) regarding Dicer antibody |
Customer FAQs regarding Dicer antibody |
Protocols for Dicer antibody |
Price and availability of products related to Dicer antibody |
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Dicer antibody - related products:
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Dicer antibody - other tools:
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Contact Abcam with a Technical Enquiry about Dicer antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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