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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Dicer antibody
See all Dicer products (11) ...
Description
Rabbit polyclonal to Dicer
Specificity
Altough 90% homologous, Dicer detection by WB was unsuccessful in human and rat ovary samples in tested conditions.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (Mouse), corresponding to amino acids FTKHQVLIMTCYVALTVLK (132-150) and QNKQILVPELCAIHPI (1017 -1033).
Positive control
mouse ovary tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 217 kDa.(Whole ovary tissue lysate is a good positive control.)
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Involvement in disease
Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:601200]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.
Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UPY3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCR1 antibody
- Dcr1 homolog (Drosophila) antibody
- Dicer 1 antibody
see all
Database links
- Entrez Gene: 23405 Human
- Entrez Gene: 192119 Mouse
- Omim: 606241 Human
- SwissProt: Q9UPY3 Human
- SwissProt: Q8R418 Mouse
- Unigene: 87889 Human
- Unigene: 21135 Mouse
Anti-Dicer antibody images:
- Dicer antibody (ab13502)
ab13502 at a 1/1000 dilution staining approximately 217kDa Dicer in 15
Western blot - Dicer antibody (ab13502)
Anti-Dicer antibody (ab13502) at 1/1000 dilution + Human HeLa cell, whole cell lysate.
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 218 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa (possible non-specific binding).
Exposure time : 3 minutes
This image is courtesy of an Abreview submitted by Dr Andrey Poleshko
Immunocytochemistry/ Immunofluorescence - Dicer antibody (ab13502)
ICC/IF image of ab13502 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13502, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Dicer antibody (ab13502)
This product has been referenced in:
- Grelier Get al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer 101:673-83 (2009). WB; Human.Read more (PubMed: 19672267) »
- Chen JFet al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A 105:2111-6 (2008). WB; Mouse.Read more (PubMed: 18256189) »
See all 6 publications for this product
Publishing research using ab13502? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"