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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Dicer antibody
See all Dicer products (11) ...
Description
Mouse polyclonal to Dicer
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Predicted to work with
a wide range of other species
Immunogen
Vector coding for a partial recombinant fusion protein (His tagged), corresponding to internal sequence amino acids 2044-2063 of Fruit fly (Drosophila melanogaster) Dicer (Dicer 1) Target sequence used to make the antibody: TRHLYEDPRQHSPGALTDLR.
TRHLYEDPRQ HSPGALTDLR
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein
Clonality
Polyclonal
Isotype
IgG
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Western blot - Dcr1 / Dicer 1 antibody (ab52680)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 255 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Involvement in disease
Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:601200]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.
Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UPY3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCR1 antibody
- Dicer 1 ribonuclease type III antibody
- Dicer antibody
see all
Database links
- Entrez Gene: 42693 Fruit fly (Drosophila melanogaster)
- SwissProt: Q9VCU9 Fruit fly (Drosophila melanogaster)
Anti-Dicer antibody images:
Western blot - Dcr1 / Dicer 1 antibody (ab52680)
All lanes : Anti-Dicer antibody (ab52680) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution
Predicted band size : 255 kDa
Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
References for Anti-Dicer antibody (ab52680)
This product has been referenced in:
- Mukherjee S & Hanley KA RNA interference modulates replication of dengue virus in Drosophila melanogaster cells. BMC Microbiol 10:127 (2010). WB; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20420715) »
See 1 publication for this product
Publishing research using ab52680? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"