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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab5818 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody (ab5818)
ab5818 (1/200) staining Dicer in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
Western blot - Dicer antibody (ab5818)
All lanes : Anti-Dicer antibody (ab5818) at 2 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 10 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 5 : HEK293 Whole Cell Lysate Overexpressing Dicer at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 217 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody (ab5818)
ICC/IF image of ab5818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5818, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Dicer antibody for Immunocytochemistry/ Immunofluorescence in Human (5818)
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